| http://purl.uniprot.org/citations/20876560 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20876560 | http://www.w3.org/2000/01/rdf-schema#comment | "Interchromosomal duplications are especially important for the study of X-linked genes. Males inheriting a mutation in a vital X-linked gene cannot survive unless there is a wild-type copy of the gene duplicated elsewhere in the genome. Rescuing the lethality of an X-linked mutation with a duplication allows the mutation to be used experimentally in complementation tests and other genetic crosses and it maps the mutated gene to a defined chromosomal region. Duplications can also be used to screen for dosage-dependent enhancers and suppressors of mutant phenotypes as a way to identify genes involved in the same biological process. We describe an ongoing project in Drosophila melanogaster to generate comprehensive coverage and extensive breakpoint subdivision of the X chromosome with megabase-scale X segments borne on Y chromosomes. The in vivo method involves the creation of X inversions on attached-XY chromosomes by FLP-FRT site-specific recombination technology followed by irradiation to induce large internal X deletions. The resulting chromosomes consist of the X tip, a medial X segment placed near the tip by an inversion, and a full Y. A nested set of medial duplicated segments is derived from each inversion precursor. We have constructed a set of inversions on attached-XY chromosomes that enable us to isolate nested duplicated segments from all X regions. To date, our screens have provided a minimum of 78% X coverage with duplication breakpoints spaced a median of nine genes apart. These duplication chromosomes will be valuable resources for rescuing and mapping X-linked mutations and identifying dosage-dependent modifiers of mutant phenotypes."xsd:string |
| http://purl.uniprot.org/citations/20876560 | http://purl.org/dc/terms/identifier | "doi:10.1534/genetics.110.123265"xsd:string |
| http://purl.uniprot.org/citations/20876560 | http://purl.uniprot.org/core/author | "Ward M.E."xsd:string |
| http://purl.uniprot.org/citations/20876560 | http://purl.uniprot.org/core/author | "Cook R.K."xsd:string |
| http://purl.uniprot.org/citations/20876560 | http://purl.uniprot.org/core/author | "Kaufman T.C."xsd:string |
| http://purl.uniprot.org/citations/20876560 | http://purl.uniprot.org/core/author | "Spana E.P."xsd:string |
| http://purl.uniprot.org/citations/20876560 | http://purl.uniprot.org/core/author | "Brown C.A."xsd:string |
| http://purl.uniprot.org/citations/20876560 | http://purl.uniprot.org/core/author | "Cook K.R."xsd:string |
| http://purl.uniprot.org/citations/20876560 | http://purl.uniprot.org/core/author | "Andrade R.S."xsd:string |
| http://purl.uniprot.org/citations/20876560 | http://purl.uniprot.org/core/author | "Deal J.A."xsd:string |
| http://purl.uniprot.org/citations/20876560 | http://purl.uniprot.org/core/author | "Deal M.E."xsd:string |
| http://purl.uniprot.org/citations/20876560 | http://purl.uniprot.org/core/author | "Garton R.D."xsd:string |
| http://purl.uniprot.org/citations/20876560 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20876560 | http://purl.uniprot.org/core/name | "Genetics"xsd:string |
| http://purl.uniprot.org/citations/20876560 | http://purl.uniprot.org/core/pages | "1095-1109"xsd:string |
| http://purl.uniprot.org/citations/20876560 | http://purl.uniprot.org/core/title | "A new resource for characterizing X-linked genes in Drosophila melanogaster: systematic coverage and subdivision of the X chromosome with nested, Y-linked duplications."xsd:string |
| http://purl.uniprot.org/citations/20876560 | http://purl.uniprot.org/core/volume | "186"xsd:string |
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