| http://purl.uniprot.org/citations/2089044 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2089044 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2089044 | http://www.w3.org/2000/01/rdf-schema#comment | "The molecular cloning and the determination of the nucleotide sequence of the ispA gene responsible for farnesyl diphosphate (FPP) synthase [EC 2.5.1.1] activity in Escherichia coli are described. E. coli ispA strains have temperature-sensitive FPP synthase, and the defective gene is located at about min 10 on the chromosome. The wild-type ispA gene was subcloned from a lambda phage clone containing the chromosomal fragment around min 10, picked up from the aligned genomic library of Kohara et al. [Kohara, Y., Akiyama, K., & Isono, K. (1987) Cell 50, 495-508]. The cloned gene was identified as the ispA gene by the recovery and amplification of FPP synthase activity in an ispA strain. A 1,452-nucleotide sequence of the cloned fragment was determined. This sequence specifies two open reading frames, ORF-1 and ORF-2, encoding proteins with the expected molecular weights of 8,951 and 32,158, respectively. A part of the deduced amino acid sequence of ORF-2 showed similarity to the sequences of eucaryotic FPP synthases and of crtE product of a photosynthetic bacterium. The plasmid carrying ORF-2 downstream of the lac promoter complemented the defect of FPP synthase activity of the ispA mutant, showing that the product encoded by ORF-2 is the ispA product. The maxicell analysis indicated that a protein of molecular weight 36,000, approximately consistent with the molecular weight of the deduced ORF-2-encoded protein, is the gene product."xsd:string |
| http://purl.uniprot.org/citations/2089044 | http://purl.org/dc/terms/identifier | "doi:10.1093/oxfordjournals.jbchem.a123327"xsd:string |
| http://purl.uniprot.org/citations/2089044 | http://purl.org/dc/terms/identifier | "doi:10.1093/oxfordjournals.jbchem.a123327"xsd:string |
| http://purl.uniprot.org/citations/2089044 | http://purl.uniprot.org/core/author | "Fujisaki S."xsd:string |
| http://purl.uniprot.org/citations/2089044 | http://purl.uniprot.org/core/author | "Fujisaki S."xsd:string |
| http://purl.uniprot.org/citations/2089044 | http://purl.uniprot.org/core/author | "Hara H."xsd:string |
| http://purl.uniprot.org/citations/2089044 | http://purl.uniprot.org/core/author | "Hara H."xsd:string |
| http://purl.uniprot.org/citations/2089044 | http://purl.uniprot.org/core/author | "Nishimura Y."xsd:string |
| http://purl.uniprot.org/citations/2089044 | http://purl.uniprot.org/core/author | "Nishimura Y."xsd:string |
| http://purl.uniprot.org/citations/2089044 | http://purl.uniprot.org/core/author | "Nishino T."xsd:string |
| http://purl.uniprot.org/citations/2089044 | http://purl.uniprot.org/core/author | "Nishino T."xsd:string |
| http://purl.uniprot.org/citations/2089044 | http://purl.uniprot.org/core/author | "Horiuchi K."xsd:string |
| http://purl.uniprot.org/citations/2089044 | http://purl.uniprot.org/core/author | "Horiuchi K."xsd:string |
| http://purl.uniprot.org/citations/2089044 | http://purl.uniprot.org/core/date | "1990"xsd:gYear |
| http://purl.uniprot.org/citations/2089044 | http://purl.uniprot.org/core/date | "1990"xsd:gYear |
| http://purl.uniprot.org/citations/2089044 | http://purl.uniprot.org/core/name | "J. Biochem."xsd:string |
| http://purl.uniprot.org/citations/2089044 | http://purl.uniprot.org/core/name | "J. Biochem."xsd:string |
| http://purl.uniprot.org/citations/2089044 | http://purl.uniprot.org/core/pages | "995-1000"xsd:string |
| http://purl.uniprot.org/citations/2089044 | http://purl.uniprot.org/core/pages | "995-1000"xsd:string |
| http://purl.uniprot.org/citations/2089044 | http://purl.uniprot.org/core/title | "Cloning and nucleotide sequence of the ispA gene responsible for farnesyl diphosphate synthase activity in Escherichia coli."xsd:string |
| http://purl.uniprot.org/citations/2089044 | http://purl.uniprot.org/core/title | "Cloning and nucleotide sequence of the ispA gene responsible for farnesyl diphosphate synthase activity in Escherichia coli."xsd:string |
| http://purl.uniprot.org/citations/2089044 | http://purl.uniprot.org/core/volume | "108"xsd:string |
| http://purl.uniprot.org/citations/2089044 | http://purl.uniprot.org/core/volume | "108"xsd:string |