| http://purl.uniprot.org/citations/20926613 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20926613 | http://www.w3.org/2000/01/rdf-schema#comment | "Central chemoreception is the mechanism by which CO(2)/pH sensors regulate breathing in response to tissue pH changes. There is compelling evidence that pH-sensitive neurons in the retrotrapezoid nucleus (RTN) are important chemoreceptors. Evidence also indicates that CO(2)/H(+)-evoked adenosine 5'-triphosphate (ATP) release in the RTN, from pH-sensitive astrocytes, contributes to chemoreception. However, mechanism(s) by which RTN astrocytes sense pH is unknown and their contribution to chemoreception remains controversial. Here, we use the brain slice preparation and a combination of patch-clamp electrophysiology and immunohistochemistry to confirm that RTN astrocytes are pH sensitive and to determine mechanisms by which they sense pH. We show that pH-sensitive RTN glia are immunoreactive for aldehyde dehydrogenase 1L1, a marker of astrocytes. In HEPES buffer the pH-sensitive current expressed by RTN astrocytes reversed near E(K(+)) (the equilibrium potential for K(+)) and was inhibited by Ba(2+) and desipramine (blocker of Kir4.1-containing channels), characteristics most consistent with heteromeric Kir4.1-Kir5.1 channels. In bicarbonate buffer, the sodium/bicarbonate cotransporter also contributed to the CO(2)/H(+)-sensitive current in RTN astrocytes. To test the hypothesis that RTN astrocytes contribute to chemoreception by a purinergic mechanism, we used fluorocitrate to selectively depolarize astrocytes while measuring neuronal activity. We found that fluorocitrate increased baseline activity and pH sensitivity of RTN neurons by a P2-receptor-dependent mechanism, suggesting that astrocytes may release ATP to activate RTN chemoreceptors. We also found in bicarbonate but not HEPES buffer that P2-receptor antagonists decreased CO(2) sensitivity of RTN neurons. We conclude that RTN astrocytes sense CO(2)/H(+) in part by inhibition of a Kir4.1-Kir5.1-like current and may provide an excitatory purinergic drive to pH-sensitive neurons."xsd:string |
| http://purl.uniprot.org/citations/20926613 | http://purl.org/dc/terms/identifier | "doi:10.1152/jn.00544.2010"xsd:string |
| http://purl.uniprot.org/citations/20926613 | http://purl.uniprot.org/core/author | "Nishiyama A."xsd:string |
| http://purl.uniprot.org/citations/20926613 | http://purl.uniprot.org/core/author | "Mulkey D.K."xsd:string |
| http://purl.uniprot.org/citations/20926613 | http://purl.uniprot.org/core/author | "Kreneisz O."xsd:string |
| http://purl.uniprot.org/citations/20926613 | http://purl.uniprot.org/core/author | "Wenker I.C."xsd:string |
| http://purl.uniprot.org/citations/20926613 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20926613 | http://purl.uniprot.org/core/name | "J Neurophysiol"xsd:string |
| http://purl.uniprot.org/citations/20926613 | http://purl.uniprot.org/core/pages | "3042-3052"xsd:string |
| http://purl.uniprot.org/citations/20926613 | http://purl.uniprot.org/core/title | "Astrocytes in the retrotrapezoid nucleus sense H+ by inhibition of a Kir4.1-Kir5.1-like current and may contribute to chemoreception by a purinergic mechanism."xsd:string |
| http://purl.uniprot.org/citations/20926613 | http://purl.uniprot.org/core/volume | "104"xsd:string |
| http://purl.uniprot.org/citations/20926613 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20926613 |
| http://purl.uniprot.org/citations/20926613 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/20926613 |
| http://purl.uniprot.org/uniprot/#_A0A8I6G3K4-mappedCitation-20926613 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20926613 |
| http://purl.uniprot.org/uniprot/#_A6JG53-mappedCitation-20926613 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20926613 |
| http://purl.uniprot.org/uniprot/#_P49655-mappedCitation-20926613 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20926613 |
| http://purl.uniprot.org/uniprot/A6JG53 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/20926613 |
| http://purl.uniprot.org/uniprot/P49655 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/20926613 |
| http://purl.uniprot.org/uniprot/A0A8I6G3K4 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/20926613 |