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http://purl.uniprot.org/citations/20937232http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/20937232http://www.w3.org/2000/01/rdf-schema#comment"

Aim

we Clone the ZNF185 gene and detect the position of ZNF185 in the mouse testis.

Methods

extracted from mouse testis RNA, by RT-PCR, and then the obtained fragment was cloned and identified; extracted from mouse liver, testis and ovary proteins were Western blot analysis; preparation of frozen sections of mouse testes, immunofluorescence techniques analysis.

Results

(1) ZNF185 gene cloning was correct. (2) Western blot showed that the most abundant in the testes ZNF185. (3) Immunofluorescence showed, ZNF185 located in Leydig cells and sperm, Leydig cells in the weak, and in round spermatids and mature sperm were highly expressed.

Conclusion

the gene cloning of ZNF185 was successful and initially proved the position of ZNF185 in the mouse testis."xsd:string
http://purl.uniprot.org/citations/20937232http://purl.uniprot.org/core/author"Gao Z.Q."xsd:string
http://purl.uniprot.org/citations/20937232http://purl.uniprot.org/core/author"Chen L.M."xsd:string
http://purl.uniprot.org/citations/20937232http://purl.uniprot.org/core/author"Liu X.Y."xsd:string
http://purl.uniprot.org/citations/20937232http://purl.uniprot.org/core/author"Yu W.J."xsd:string
http://purl.uniprot.org/citations/20937232http://purl.uniprot.org/core/author"Pan Z.F."xsd:string
http://purl.uniprot.org/citations/20937232http://purl.uniprot.org/core/author"Lian B."xsd:string
http://purl.uniprot.org/citations/20937232http://purl.uniprot.org/core/author"Zhao C.L."xsd:string
http://purl.uniprot.org/citations/20937232http://purl.uniprot.org/core/author"Feng W.G."xsd:string
http://purl.uniprot.org/citations/20937232http://purl.uniprot.org/core/date"2010"xsd:gYear
http://purl.uniprot.org/citations/20937232http://purl.uniprot.org/core/name"Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi"xsd:string
http://purl.uniprot.org/citations/20937232http://purl.uniprot.org/core/pages"973-975"xsd:string
http://purl.uniprot.org/citations/20937232http://purl.uniprot.org/core/title"[ZNF185 gene cloning and the localization in mouse testis]."xsd:string
http://purl.uniprot.org/citations/20937232http://purl.uniprot.org/core/volume"26"xsd:string
http://purl.uniprot.org/citations/20937232http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/20937232
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