| http://purl.uniprot.org/citations/20937809 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20937809 | http://www.w3.org/2000/01/rdf-schema#comment | "Dynamic actin polymerization drives a variety of morphogenetic events during metazoan development. Members of the WASP/WAVE protein family are central nucleation-promoting factors. They are embedded within regulatory networks of macromolecular complexes controlling Arp2/3-mediated actin nucleation in time and space. WAVE (Wiskott-Aldrich syndrome protein family verprolin-homologous protein) proteins are found in a conserved pentameric heterocomplex that contains Abi, Kette/Nap1, Sra-1/CYFIP, and HSPC300. Formation of the WAVE complex contributes to the localization, activity, and stability of the various WAVE proteins. Here, we established the Bimolecular Fluorescence Complementation (BiFC) technique in Drosophila to determine the subcellular localization of the WAVE complex in living flies. Using different split-YFP combinations, we are able to visualize the formation of the WAVE-Abi complex in vivo. We found that WAVE also forms dimers that are capable of forming higher order clusters with endogenous WAVE complex components. The N-terminal WAVE homology domain (WHD) of the WAVE protein mediates both WAVE-Abi and WAVE-WAVE interactions. Detailed localization analyses show that formation of WAVE complexes specifically takes place at basal cell compartments promoting actin polymerization. In the wing epithelium, hetero- and homooligomeric WAVE complexes co-localize with Integrin and Talin suggesting a role in integrin-mediated cell adhesion. RNAi mediated suppression of single components of the WAVE and the Arp2/3 complex in the wing further suggests that WAVE-dependent Arp2/3-mediated actin nucleation is important for the maintenance of stable integrin junctions."xsd:string |
| http://purl.uniprot.org/citations/20937809 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m110.139337"xsd:string |
| http://purl.uniprot.org/citations/20937809 | http://purl.uniprot.org/core/author | "Bogdan S."xsd:string |
| http://purl.uniprot.org/citations/20937809 | http://purl.uniprot.org/core/author | "Banovic D."xsd:string |
| http://purl.uniprot.org/citations/20937809 | http://purl.uniprot.org/core/author | "Gohl C."xsd:string |
| http://purl.uniprot.org/citations/20937809 | http://purl.uniprot.org/core/author | "Grevelhorster A."xsd:string |
| http://purl.uniprot.org/citations/20937809 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20937809 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/20937809 | http://purl.uniprot.org/core/pages | "40171-40179"xsd:string |
| http://purl.uniprot.org/citations/20937809 | http://purl.uniprot.org/core/title | "WAVE forms hetero- and homo-oligomeric complexes at integrin junctions in Drosophila visualized by bimolecular fluorescence complementation."xsd:string |
| http://purl.uniprot.org/citations/20937809 | http://purl.uniprot.org/core/volume | "285"xsd:string |
| http://purl.uniprot.org/citations/20937809 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20937809 |
| http://purl.uniprot.org/citations/20937809 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/20937809 |
| http://purl.uniprot.org/uniprot/Q9VF87#attribution-3C81BCF408F6E5EF9CF0BEE3D62ED7EB | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/20937809 |
| http://purl.uniprot.org/uniprot/P55162#attribution-3C81BCF408F6E5EF9CF0BEE3D62ED7EB | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/20937809 |
| http://purl.uniprot.org/uniprot/Q9VMH2#attribution-3C81BCF408F6E5EF9CF0BEE3D62ED7EB | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/20937809 |
| http://purl.uniprot.org/uniprot/O97182#attribution-3C81BCF408F6E5EF9CF0BEE3D62ED7EB | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/20937809 |
| http://purl.uniprot.org/uniprot/F2FB81#attribution-3C81BCF408F6E5EF9CF0BEE3D62ED7EB | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/20937809 |
| http://purl.uniprot.org/uniprot/F2FB81#attribution-C1DEDF6E2E58E2E1C389377CA52BB5EA | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/20937809 |
| http://purl.uniprot.org/uniprot/A0A0B4KH51#attribution-3C81BCF408F6E5EF9CF0BEE3D62ED7EB | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/20937809 |
| http://purl.uniprot.org/uniprot/A0A0B4KH51#attribution-C1DEDF6E2E58E2E1C389377CA52BB5EA | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/20937809 |
| http://purl.uniprot.org/uniprot/Q9Y0S9#attribution-3C81BCF408F6E5EF9CF0BEE3D62ED7EB | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/20937809 |
| http://purl.uniprot.org/uniprot/Q9Y0S9#attribution-C1DEDF6E2E58E2E1C389377CA52BB5EA | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/20937809 |
| http://purl.uniprot.org/uniprot/H5V8D0#attribution-3C81BCF408F6E5EF9CF0BEE3D62ED7EB | http://purl.uniprot.org/core/source | http://purl.uniprot.org/citations/20937809 |