| http://purl.uniprot.org/citations/20952260 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20952260 | http://www.w3.org/2000/01/rdf-schema#comment | "The efficiency and fidelity of nucleotide incorporation and next-base extension by DNA polymerase (pol) κ past N(2)-ethyl-Gua were measured using steady-state and rapid kinetic analyses. DNA pol κ incorporated nucleotides and extended 3' termini opposite N(2)-ethyl-Gua with measured efficiencies and fidelities similar to that opposite Gua indicating a role for DNA pol κ at the insertion and extension steps of N(2)-ethyl-Gua bypass. The DNA pol κ was maximally activated to similar levels by a twenty-fold lower concentration of Mn(2+) compared to Mg(2+). In addition, the steady state analysis indicated that high fidelity DNA pol κ-catalyzed N(2)-ethyl-Gua bypass is Mg(2+)-dependent. Strikingly, Mn(2+) activation of DNA pol κ resulted in a dramatically lower efficiency of correct nucleotide incorporation opposite both N(2)-ethyl-Gua and Gua compared to that detected upon Mg(2+) activation. This effect is largely governed by diminished correct nucleotide binding as indicated by the high K(m) values for dCTP insertion opposite N(2)-ethyl-Gua and Gua with Mn(2+) activation. A rapid kinetic analysis showed diminished burst amplitudes in the presence of Mn(2+) compared to Mg(2+) indicating that DNA pol κ preferentially utilizes Mg(2+) activation. These kinetic data support a DNA pol κ wobble base pairing mechanism for dCTP incorporation opposite N(2)-ethyl-Gua. Furthermore, the dramatically different polymerization efficiencies of the Y-family DNA pols κ and ι in the presence of Mn(2+) suggest a metal ion-dependent regulation in coordinating the activities of these DNA pols during translesion synthesis."xsd:string |
| http://purl.uniprot.org/citations/20952260 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.dnarep.2010.09.007"xsd:string |
| http://purl.uniprot.org/citations/20952260 | http://purl.uniprot.org/core/author | "Perrino F.W."xsd:string |
| http://purl.uniprot.org/citations/20952260 | http://purl.uniprot.org/core/author | "Blans P."xsd:string |
| http://purl.uniprot.org/citations/20952260 | http://purl.uniprot.org/core/author | "Fishbein J.C."xsd:string |
| http://purl.uniprot.org/citations/20952260 | http://purl.uniprot.org/core/author | "Pence M.G."xsd:string |
| http://purl.uniprot.org/citations/20952260 | http://purl.uniprot.org/core/author | "Zink C.N."xsd:string |
| http://purl.uniprot.org/citations/20952260 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/20952260 | http://purl.uniprot.org/core/name | "DNA Repair (Amst)"xsd:string |
| http://purl.uniprot.org/citations/20952260 | http://purl.uniprot.org/core/pages | "56-64"xsd:string |
| http://purl.uniprot.org/citations/20952260 | http://purl.uniprot.org/core/title | "Bypass of N^2-ethylguanine by human DNA polymerase kappa."xsd:string |
| http://purl.uniprot.org/citations/20952260 | http://purl.uniprot.org/core/volume | "10"xsd:string |
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