| http://purl.uniprot.org/citations/20952576 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20952576 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20952576 | http://www.w3.org/2000/01/rdf-schema#comment | "Cytochrome oxidases are perfect model substrates for analyzing the assembly of multisubunit complexes because the need for cofactor incorporation adds an additional level of complexity to their assembly. cbb(3)-type cytochrome c oxidases (cbb(3)-Cox) consist of the catalytic subunit CcoN, the membrane-bound c-type cytochrome subunits CcoO and CcoP, and the CcoQ subunit, which is required for cbb(3)-Cox stability. Biogenesis of cbb(3)-Cox proceeds via CcoQP and CcoNO subcomplexes, which assemble into the active cbb(3)-Cox. Most bacteria expressing cbb(3)-Cox also contain the ccoGHIS genes, which encode putative cbb(3)-Cox assembly factors. Their exact function, however, has remained unknown. Here we analyzed the role of CcoH in cbb(3)-Cox assembly and showed that CcoH is a single spanning-membrane protein with an N-terminus-out-C-terminus-in (N(out)-C(in)) topology. In its absence, neither the fully assembled cbb(3)-Cox nor the CcoQP or CcoNO subcomplex was detectable. By chemical cross-linking, we demonstrated that CcoH binds primarily via its transmembrane domain to the CcoP subunit of cbb(3)-Cox. A second hydrophobic stretch, which is located at the C terminus of CcoH, appears not to be required for contacting CcoP, but deleting it prevents the formation of the active cbb(3)-Cox. This suggests that the second hydrophobic domain is required for merging the CcoNO and CcoPQ subcomplexes into the active cbb(3)-Cox. Surprisingly, CcoH does not seem to interact only transiently with the cbb(3)-Cox but appears to stay tightly associated with the active, fully assembled complex. Thus, CcoH behaves more like a bona fide subunit of the cbb(3)-Cox than an assembly factor per se."xsd:string |
| http://purl.uniprot.org/citations/20952576 | http://purl.org/dc/terms/identifier | "doi:10.1128/jb.00988-10"xsd:string |
| http://purl.uniprot.org/citations/20952576 | http://purl.org/dc/terms/identifier | "doi:10.1128/jb.00988-10"xsd:string |
| http://purl.uniprot.org/citations/20952576 | http://purl.uniprot.org/core/author | "Waidner B."xsd:string |
| http://purl.uniprot.org/citations/20952576 | http://purl.uniprot.org/core/author | "Waidner B."xsd:string |
| http://purl.uniprot.org/citations/20952576 | http://purl.uniprot.org/core/author | "Daldal F."xsd:string |
| http://purl.uniprot.org/citations/20952576 | http://purl.uniprot.org/core/author | "Daldal F."xsd:string |
| http://purl.uniprot.org/citations/20952576 | http://purl.uniprot.org/core/author | "Hellwig P."xsd:string |
| http://purl.uniprot.org/citations/20952576 | http://purl.uniprot.org/core/author | "Hellwig P."xsd:string |
| http://purl.uniprot.org/citations/20952576 | http://purl.uniprot.org/core/author | "Koch H.G."xsd:string |
| http://purl.uniprot.org/citations/20952576 | http://purl.uniprot.org/core/author | "Koch H.G."xsd:string |
| http://purl.uniprot.org/citations/20952576 | http://purl.uniprot.org/core/author | "Kulajta C."xsd:string |
| http://purl.uniprot.org/citations/20952576 | http://purl.uniprot.org/core/author | "Kulajta C."xsd:string |
| http://purl.uniprot.org/citations/20952576 | http://purl.uniprot.org/core/author | "Pawlik G."xsd:string |
| http://purl.uniprot.org/citations/20952576 | http://purl.uniprot.org/core/author | "Pawlik G."xsd:string |
| http://purl.uniprot.org/citations/20952576 | http://purl.uniprot.org/core/author | "Sachelaru I."xsd:string |
| http://purl.uniprot.org/citations/20952576 | http://purl.uniprot.org/core/author | "Sachelaru I."xsd:string |
| http://purl.uniprot.org/citations/20952576 | http://purl.uniprot.org/core/author | "Schroder S."xsd:string |
| http://purl.uniprot.org/citations/20952576 | http://purl.uniprot.org/core/author | "Schroder S."xsd:string |
| http://purl.uniprot.org/citations/20952576 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20952576 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20952576 | http://purl.uniprot.org/core/name | "J. Bacteriol."xsd:string |
| http://purl.uniprot.org/citations/20952576 | http://purl.uniprot.org/core/name | "J. Bacteriol."xsd:string |