| http://purl.uniprot.org/citations/20971846 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20971846 | http://www.w3.org/2000/01/rdf-schema#comment | "RasC is required for optimum activation of adenylyl cyclase A and for aggregate stream formation during the early differentiation of Dictyostelium discoideum. RasG is unable to substitute for this requirement despite its sequence similarity to RasC. A critical question is which amino acids in RasC are required for its specific function. Each of the amino acids within the switch 1 and 2 domains in the N-terminal portion of RasG was changed to the corresponding amino acid from RasC, and the ability of the mutated RasG protein to reverse the phenotype of rasC(-) cells was determined. Only the change from aspartate at position 30 of RasG to alanine (the equivalent position 31 in RasC) resulted in a significant increase in adenylyl cyclase A activation and a partial reversal of the aggregation-deficient phenotype of rasC(-) cells. All other single amino acid changes were without effect. Expression of a chimeric protein, RasG(1-77)-RasC(79-189), also resulted in a partial reversal of the rasC(-) cell phenotype, indicating the importance of the C-terminal portion of RasC. Furthermore, expression of the chimeric protein, with alanine changed to aspartate (RasG(1-77(D30A))-RasC(79-189)), resulted in a full rescue the rasC(-) aggregation-deficient phenotype. Finally, the expression of either a mutated RasC, with the aspartate 31 replaced by alanine, or the chimeric protein, RasC(1-78)-RasG(78-189), only generated a partial rescue. These results emphasize the importance of both the single amino acid at position 31 and the C-terminal sequence for the specific function of RasC during Dictyostelium aggregation."xsd:string |
| http://purl.uniprot.org/citations/20971846 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m110.181115"xsd:string |
| http://purl.uniprot.org/citations/20971846 | http://purl.uniprot.org/core/author | "Weeks G."xsd:string |
| http://purl.uniprot.org/citations/20971846 | http://purl.uniprot.org/core/author | "Bolourani P."xsd:string |
| http://purl.uniprot.org/citations/20971846 | http://purl.uniprot.org/core/author | "Spiegelman G."xsd:string |
| http://purl.uniprot.org/citations/20971846 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20971846 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/20971846 | http://purl.uniprot.org/core/pages | "41374-41379"xsd:string |
| http://purl.uniprot.org/citations/20971846 | http://purl.uniprot.org/core/title | "Determinants of RasC specificity during Dictyostelium aggregation."xsd:string |
| http://purl.uniprot.org/citations/20971846 | http://purl.uniprot.org/core/volume | "285"xsd:string |
| http://purl.uniprot.org/citations/20971846 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20971846 |
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| http://purl.uniprot.org/uniprot/#_P15064-mappedCitation-20971846 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20971846 |
| http://purl.uniprot.org/uniprot/#_Q03100-mappedCitation-20971846 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20971846 |
| http://purl.uniprot.org/uniprot/P32253 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/20971846 |
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| http://purl.uniprot.org/uniprot/P15064 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/20971846 |