| http://purl.uniprot.org/citations/20974745 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/20974745 | http://www.w3.org/2000/01/rdf-schema#comment | "We describe methods for obtaining a quantitative description of RNA processing at high resolution in budding yeast. As a model gene expression system, we constructed tetON (for induction studies) and tetOFF (for repression, derepression, and RNA degradation studies) yeast strains with a series of reporter genes integrated in the genome under the control of a tetO7 promoter. Reverse transcription and quantitative real-time-PCR (RT-qPCR) methods were adapted to allow the determination of mRNA abundance as the average number of copies per cell in a population. Fluorescence in situ hybridization (FISH) measurements of transcript numbers in individual cells validated the RT-qPCR approach for the average copy-number determination despite the broad distribution of transcript levels within a population of cells. In addition, RT-qPCR was used to distinguish the products of the different steps in splicing of the reporter transcripts, and methods were developed to map and quantify 3'-end cleavage and polyadenylation. This system permits pre-mRNA production, splicing, 3'-end maturation and degradation to be quantitatively monitored with unprecedented kinetic detail, suitable for mathematical modeling. Using this approach, we demonstrate that reporter transcripts are spliced prior to their 3'-end cleavage and polyadenylation, that is, cotranscriptionally."xsd:string |
| http://purl.uniprot.org/citations/20974745 | http://purl.org/dc/terms/identifier | "doi:10.1261/rna.2162610"xsd:string |
| http://purl.uniprot.org/citations/20974745 | http://purl.uniprot.org/core/author | "Beggs J.D."xsd:string |
| http://purl.uniprot.org/citations/20974745 | http://purl.uniprot.org/core/author | "Tollervey D."xsd:string |
| http://purl.uniprot.org/citations/20974745 | http://purl.uniprot.org/core/author | "Bertrand E."xsd:string |
| http://purl.uniprot.org/citations/20974745 | http://purl.uniprot.org/core/author | "Dichtl B."xsd:string |
| http://purl.uniprot.org/citations/20974745 | http://purl.uniprot.org/core/author | "Barrass J.D."xsd:string |
| http://purl.uniprot.org/citations/20974745 | http://purl.uniprot.org/core/author | "Kos M."xsd:string |
| http://purl.uniprot.org/citations/20974745 | http://purl.uniprot.org/core/author | "Kufel J."xsd:string |
| http://purl.uniprot.org/citations/20974745 | http://purl.uniprot.org/core/author | "Mariconti L."xsd:string |
| http://purl.uniprot.org/citations/20974745 | http://purl.uniprot.org/core/author | "Karkusiewicz I."xsd:string |
| http://purl.uniprot.org/citations/20974745 | http://purl.uniprot.org/core/author | "Robert M.C."xsd:string |
| http://purl.uniprot.org/citations/20974745 | http://purl.uniprot.org/core/author | "Koper M."xsd:string |
| http://purl.uniprot.org/citations/20974745 | http://purl.uniprot.org/core/author | "Alexander R.D."xsd:string |
| http://purl.uniprot.org/citations/20974745 | http://purl.uniprot.org/core/author | "Obtulowicz T."xsd:string |
| http://purl.uniprot.org/citations/20974745 | http://purl.uniprot.org/core/author | "Dichtl B.'"xsd:string |
| http://purl.uniprot.org/citations/20974745 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/20974745 | http://purl.uniprot.org/core/name | "RNA"xsd:string |
| http://purl.uniprot.org/citations/20974745 | http://purl.uniprot.org/core/pages | "2570-2580"xsd:string |
| http://purl.uniprot.org/citations/20974745 | http://purl.uniprot.org/core/title | "RiboSys, a high-resolution, quantitative approach to measure the in vivo kinetics of pre-mRNA splicing and 3'-end processing in Saccharomyces cerevisiae."xsd:string |
| http://purl.uniprot.org/citations/20974745 | http://purl.uniprot.org/core/volume | "16"xsd:string |
| http://purl.uniprot.org/citations/20974745 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/20974745 |
| http://purl.uniprot.org/citations/20974745 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/20974745 |
| http://purl.uniprot.org/uniprot/#_P00560-mappedCitation-20974745 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/20974745 |