DESCRIBE <http://purl.uniprot.org/citations/20980461>

Subject	Predicate	Object
http://purl.uniprot.org/citations/20980461	http://www.w3.org/1999/02/22-rdf-syntax-ns#type	http://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/20980461	http://www.w3.org/2000/01/rdf-schema#comment	" Objective The agouti-related protein (Agrp) is a powerful orexigenic peptide, but little is known about its transcriptional regulation. The objective of this study was to determine molecular mechanisms for the activation of hypothalamic Agrp and identify compounds that stimulate appetite. Research design and methods We used promoter analyses methods, hypothalamic cell culture and transfection, immunohistochemistry, luciferase-expressing transgenic mice, in vivo bioluminescence, anitisense RNA, mouse feeding studies, indirect calorimetry, real-time PCR, and Western blots. Results We found that the Krüppel-like factor 4 (Klf4) is a potent activator of Agrp by binding to a specific CACCC-box in its minimal promoter. We also found that an extract of tarragon, termed PMI-5011, activated hypothalamic Klf4 and Agrp. In vivo, PMI-5011 increased Agrp promoter activity in luciferase-expressing transgenic mice, increased hypothalamic Klf4 and Agrp expression, increased hypothalamic Orexin and melanin-concentrating hormone, increased food intake, reduced circulating insulin and leptin levels, attenuated energy expenditure, and enhanced body weight but only when using a high-fat diet. Conclusions These data show that Klf4 augmented hypothalamic Agrp by binding to a specific CACCC-box onto its minimal promoter. In addition, the tarragon extract PMI-5011 activated Klf4 and orexigenic neuropeptides and reduced peripheral insulin and leptin levels leading to positive energy balance."xsd:string
http://purl.uniprot.org/citations/20980461	http://purl.org/dc/terms/identifier	"doi:10.2337/db10-0172"xsd:string
http://purl.uniprot.org/citations/20980461	http://purl.uniprot.org/core/author	"Argyropoulos G."xsd:string
http://purl.uniprot.org/citations/20980461	http://purl.uniprot.org/core/author	"Zuberi A."xsd:string
http://purl.uniprot.org/citations/20980461	http://purl.uniprot.org/core/author	"Cefalu W.T."xsd:string
http://purl.uniprot.org/citations/20980461	http://purl.uniprot.org/core/author	"Ilnytska O."xsd:string
http://purl.uniprot.org/citations/20980461	http://purl.uniprot.org/core/author	"Stutz A.M."xsd:string
http://purl.uniprot.org/citations/20980461	http://purl.uniprot.org/core/author	"York D.A."xsd:string
http://purl.uniprot.org/citations/20980461	http://purl.uniprot.org/core/author	"Park-York M."xsd:string
http://purl.uniprot.org/citations/20980461	http://purl.uniprot.org/core/author	"Ribnicky D.M."xsd:string
http://purl.uniprot.org/citations/20980461	http://purl.uniprot.org/core/date	"2011"xsd:gYear
http://purl.uniprot.org/citations/20980461	http://purl.uniprot.org/core/name	"Diabetes"xsd:string
http://purl.uniprot.org/citations/20980461	http://purl.uniprot.org/core/pages	"97-106"xsd:string
http://purl.uniprot.org/citations/20980461	http://purl.uniprot.org/core/title	"Molecular mechanisms for activation of the agouti-related protein and stimulation of appetite."xsd:string
http://purl.uniprot.org/citations/20980461	http://purl.uniprot.org/core/volume	"60"xsd:string
http://purl.uniprot.org/citations/20980461	http://www.w3.org/2004/02/skos/core#exactMatch	http://purl.uniprot.org/pubmed/20980461
http://purl.uniprot.org/citations/20980461	http://xmlns.com/foaf/0.1/primaryTopicOf	https://pubmed.ncbi.nlm.nih.gov/20980461
http://purl.uniprot.org/uniprot/#_A0A0A6YX09-mappedCitation-20980461	http://www.w3.org/1999/02/22-rdf-syntax-ns#object	http://purl.uniprot.org/citations/20980461
http://purl.uniprot.org/uniprot/#_B7ZCH1-mappedCitation-20980461	http://www.w3.org/1999/02/22-rdf-syntax-ns#object	http://purl.uniprot.org/citations/20980461
http://purl.uniprot.org/uniprot/#_B7ZCH2-mappedCitation-20980461	http://www.w3.org/1999/02/22-rdf-syntax-ns#object	http://purl.uniprot.org/citations/20980461
http://purl.uniprot.org/uniprot/#_P56473-mappedCitation-20980461	http://www.w3.org/1999/02/22-rdf-syntax-ns#object	http://purl.uniprot.org/citations/20980461
http://purl.uniprot.org/uniprot/#_Q3UU47-mappedCitation-20980461	http://www.w3.org/1999/02/22-rdf-syntax-ns#object	http://purl.uniprot.org/citations/20980461
http://purl.uniprot.org/uniprot/#_Q5J6B1-mappedCitation-20980461	http://www.w3.org/1999/02/22-rdf-syntax-ns#object	http://purl.uniprot.org/citations/20980461
http://purl.uniprot.org/uniprot/#_Q60793-mappedCitation-20980461	http://www.w3.org/1999/02/22-rdf-syntax-ns#object	http://purl.uniprot.org/citations/20980461