http://purl.uniprot.org/citations/21031264 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
http://purl.uniprot.org/citations/21031264 | http://www.w3.org/2000/01/rdf-schema#comment | "The functional significance of tyrosine 207 of Saccharomyces cerevisiae phosphoenolpyruvate carboxykinase was explored by examining the kinetic properties of the Tyr207Leu mutant. The variant enzyme retained the structural characteristics of the wild-type protein as indicated by circular dichroism, intrinsic fluorescence spectroscopy, and gel-exclusion chromatography. Kinetic analyses of the mutated variant showed a 15-fold increase in K(m)CO₂, a 32-fold decrease in V(max), and a 6-fold decrease in K(m) for phosphoenolpyruvate. These results suggest that the hydroxyl group of Tyr 207 may polarize CO₂ and oxaloacetate, thus facilitating the carboxylation/decarboxylation steps."xsd:string |
http://purl.uniprot.org/citations/21031264 | http://purl.uniprot.org/core/author | "Cardemil E."xsd:string |
http://purl.uniprot.org/citations/21031264 | http://purl.uniprot.org/core/author | "Sepulveda C."xsd:string |
http://purl.uniprot.org/citations/21031264 | http://purl.uniprot.org/core/author | "Jabalquinto A.M."xsd:string |
http://purl.uniprot.org/citations/21031264 | http://purl.uniprot.org/core/author | "Andrade C."xsd:string |
http://purl.uniprot.org/citations/21031264 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
http://purl.uniprot.org/citations/21031264 | http://purl.uniprot.org/core/name | "Biol Res"xsd:string |
http://purl.uniprot.org/citations/21031264 | http://purl.uniprot.org/core/pages | "191-195"xsd:string |
http://purl.uniprot.org/citations/21031264 | http://purl.uniprot.org/core/title | "The role of tyrosine 207 in the reaction catalyzed by Saccharomyces cerevisiae phosphoenolpyruvate carboxykinase."xsd:string |
http://purl.uniprot.org/citations/21031264 | http://purl.uniprot.org/core/volume | "43"xsd:string |
http://purl.uniprot.org/citations/21031264 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/21031264 |
http://purl.uniprot.org/citations/21031264 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/21031264 |
http://purl.uniprot.org/uniprot/#_P10963-mappedCitation-21031264 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21031264 |
http://purl.uniprot.org/uniprot/P10963 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/21031264 |