| http://purl.uniprot.org/citations/21057870 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21057870 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21057870 | http://www.w3.org/2000/01/rdf-schema#comment | "Human β-1,4-galactosyltransferase (β-1,4-GalT) V was shown to be involved in the biosynthesis of N-glycans, O-glycans and lactosylceramide (Lac-Cer) by in vitro studies. To determine its substrate specificity, enzymatic activity and its products were analyzed using mouse embryonic fibroblast (MEF) cells from β-1,4-GalT V (B4galt5)-mutant mice. Analysis of expression levels of the β-1,4-GalT I-VI genes revealed that the expression of the β-1,4-GalT V gene in B4galt5 ( +/-) - and B4galt5 ( -/-) -derived MEF cells are a half and null when compared to that of B4galt5 ( +/+ )-derived MEF cells without altering the expression levels of other β-1,4-GalT genes. These MEF cells showed no apparent difference in their growth. When β-1,4-GalT activities were determined towards GlcNAcβ-S-pNP, no significant difference in its specific activity was obtained among B4galt5 ( +/+ )-, B4galt5 ( +/- ) - and B4galt5 ( -/-) -derived MEF cells. No significant differences were obtained in structures and amounts of N-glycans and lectin bindings to membrane glycoproteins among B4galt5 ( +/+ )-, B4galt5 ( +/- ) - and B4galt5 ( -/-) -derived MEF cells. However, when cell homogenates were incubated with glucosylceramide in the presence of UDP-[(3)H]Gal, Lac-Cer synthase activity in B4galt5 ( +/- ) - and B4galt5 ( -/-) -derived MEF cells decreased to 41% and 11% of that of B4galt5 ( +/+ )-derived MEF cells. Consistent with this, amounts of Lac-Cer and its derivative GM3 in B4galt5 ( -/-) -derived MEF cells decreased remarkably when compared with those of B4galt5 ( +/+ )-derived MEF cells. These results indicate that murine β-1,4-GalT V is involved in Lac-Cer biosynthesis."xsd:string |
| http://purl.uniprot.org/citations/21057870 | http://purl.org/dc/terms/identifier | "doi:10.1007/s10719-010-9313-2"xsd:string |
| http://purl.uniprot.org/citations/21057870 | http://purl.org/dc/terms/identifier | "doi:10.1007/s10719-010-9313-2"xsd:string |
| http://purl.uniprot.org/citations/21057870 | http://purl.uniprot.org/core/author | "Kumagai T."xsd:string |
| http://purl.uniprot.org/citations/21057870 | http://purl.uniprot.org/core/author | "Kumagai T."xsd:string |
| http://purl.uniprot.org/citations/21057870 | http://purl.uniprot.org/core/author | "Kobayashi Y."xsd:string |
| http://purl.uniprot.org/citations/21057870 | http://purl.uniprot.org/core/author | "Kobayashi Y."xsd:string |
| http://purl.uniprot.org/citations/21057870 | http://purl.uniprot.org/core/author | "Furukawa K."xsd:string |
| http://purl.uniprot.org/citations/21057870 | http://purl.uniprot.org/core/author | "Furukawa K."xsd:string |
| http://purl.uniprot.org/citations/21057870 | http://purl.uniprot.org/core/author | "Sato T."xsd:string |
| http://purl.uniprot.org/citations/21057870 | http://purl.uniprot.org/core/author | "Sato T."xsd:string |
| http://purl.uniprot.org/citations/21057870 | http://purl.uniprot.org/core/author | "Zhou D."xsd:string |
| http://purl.uniprot.org/citations/21057870 | http://purl.uniprot.org/core/author | "Zhou D."xsd:string |
| http://purl.uniprot.org/citations/21057870 | http://purl.uniprot.org/core/author | "Hayakawa S."xsd:string |
| http://purl.uniprot.org/citations/21057870 | http://purl.uniprot.org/core/author | "Hayakawa S."xsd:string |
| http://purl.uniprot.org/citations/21057870 | http://purl.uniprot.org/core/author | "Natsuka S."xsd:string |
| http://purl.uniprot.org/citations/21057870 | http://purl.uniprot.org/core/author | "Natsuka S."xsd:string |
| http://purl.uniprot.org/citations/21057870 | http://purl.uniprot.org/core/author | "Shinkai T."xsd:string |
| http://purl.uniprot.org/citations/21057870 | http://purl.uniprot.org/core/author | "Shinkai T."xsd:string |
| http://purl.uniprot.org/citations/21057870 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/21057870 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/21057870 | http://purl.uniprot.org/core/name | "Glycoconj. J."xsd:string |
| http://purl.uniprot.org/citations/21057870 | http://purl.uniprot.org/core/name | "Glycoconj. J."xsd:string |