| http://purl.uniprot.org/citations/21106003 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21106003 | http://www.w3.org/2000/01/rdf-schema#comment | "High-resolution melting (HRM) analysis relies on the use of fluorescent dyes, such as LCGreen, ResoLight, and SYTO9, which bind in a saturated manner to the double-stranded DNAs. These dyes are expensive in use and may not be affordable when dealing with a large quantity of samples. EvaGreen is a much cheaper DNA helix intercalating dye and has been used in quantitative real-time polymerase chain reaction (PCR) and post-PCR DNA melt curve analysis. Here we report on the development of an EvaGreen-based HRM analysis and its performance, in comparison with the popular LCGreen-based HRM analysis, in detection of DNA polymorphism in plants. We found that various polymorphisms ranged from single nucleotide polymorphisms (SNPs) to Indels were equally detected by using EvaGreen- or LCGreen-based HRM. EvaGreen dye was sensitive enough in discovery of SNPs in fivefold pooled samples. Using this economical dye we successfully identified multiple novel mutant alleles of Gln1-3 gene, which produces a cytosolic glutamine synthetase isoenzyme (GS1), in a maize ethyl methanesulfonate (EMS)-mutagenized library, and genotyped rice mapping populations with SNP markers. The current results suggest that EvaGreen is a promising dye for HRM analysis for its ease to use and cost effectiveness."xsd:string |
| http://purl.uniprot.org/citations/21106003 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1744-7909.2010.01001.x"xsd:string |
| http://purl.uniprot.org/citations/21106003 | http://purl.uniprot.org/core/author | "Liu Y.G."xsd:string |
| http://purl.uniprot.org/citations/21106003 | http://purl.uniprot.org/core/author | "Jing H.C."xsd:string |
| http://purl.uniprot.org/citations/21106003 | http://purl.uniprot.org/core/author | "Liu X.G."xsd:string |
| http://purl.uniprot.org/citations/21106003 | http://purl.uniprot.org/core/author | "Hao D.Y."xsd:string |
| http://purl.uniprot.org/citations/21106003 | http://purl.uniprot.org/core/author | "Li Y.D."xsd:string |
| http://purl.uniprot.org/citations/21106003 | http://purl.uniprot.org/core/author | "Chu Z.Z."xsd:string |
| http://purl.uniprot.org/citations/21106003 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/21106003 | http://purl.uniprot.org/core/name | "J Integr Plant Biol"xsd:string |
| http://purl.uniprot.org/citations/21106003 | http://purl.uniprot.org/core/pages | "1036-1042"xsd:string |
| http://purl.uniprot.org/citations/21106003 | http://purl.uniprot.org/core/title | "A cost-effective high-resolution melting approach using the EvaGreen dye for DNA polymorphism detection and genotyping in plants."xsd:string |
| http://purl.uniprot.org/citations/21106003 | http://purl.uniprot.org/core/volume | "52"xsd:string |
| http://purl.uniprot.org/citations/21106003 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/21106003 |
| http://purl.uniprot.org/citations/21106003 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/21106003 |
| http://purl.uniprot.org/uniprot/#_P14656-mappedCitation-21106003 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21106003 |
| http://purl.uniprot.org/uniprot/#_Q9FE01-mappedCitation-21106003 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21106003 |
| http://purl.uniprot.org/uniprot/Q9FE01 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/21106003 |
| http://purl.uniprot.org/uniprot/P14656 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/21106003 |