| http://purl.uniprot.org/citations/21107322 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21107322 | http://www.w3.org/2000/01/rdf-schema#comment | "The ubiquitylation of cell-cycle regulatory proteins by the large multimeric anaphase-promoting complex (APC/C) controls sister chromatid segregation and the exit from mitosis. Selection of APC/C targets is achieved through recognition of destruction motifs, predominantly the destruction (D)-box and KEN (Lys-Glu-Asn)-box. Although this process is known to involve a co-activator protein (either Cdc20 or Cdh1) together with core APC/C subunits, the structural basis for substrate recognition and ubiquitylation is not understood. Here we investigate budding yeast APC/C using single-particle electron microscopy and determine a cryo-electron microscopy map of APC/C in complex with the Cdh1 co-activator protein (APC/C(Cdh1)) bound to a D-box peptide at ∼10 Å resolution. We find that a combined catalytic and substrate-recognition module is located within the central cavity of the APC/C assembled from Cdh1, Apc10--a core APC/C subunit previously implicated in substrate recognition--and the cullin domain of Apc2. Cdh1 and Apc10, identified from difference maps, create a co-receptor for the D-box following repositioning of Cdh1 towards Apc10. Using NMR spectroscopy we demonstrate specific D-box-Apc10 interactions, consistent with a role for Apc10 in directly contributing towards D-box recognition by the APC/C(Cdh1) complex. Our results rationalize the contribution of both co-activator and core APC/C subunits to D-box recognition and provide a structural framework for understanding mechanisms of substrate recognition and catalysis by the APC/C."xsd:string |
| http://purl.uniprot.org/citations/21107322 | http://purl.org/dc/terms/identifier | "doi:10.1038/nature09625"xsd:string |
| http://purl.uniprot.org/citations/21107322 | http://purl.uniprot.org/core/author | "Barford D."xsd:string |
| http://purl.uniprot.org/citations/21107322 | http://purl.uniprot.org/core/author | "Zhang Z."xsd:string |
| http://purl.uniprot.org/citations/21107322 | http://purl.uniprot.org/core/author | "Williams M.A."xsd:string |
| http://purl.uniprot.org/citations/21107322 | http://purl.uniprot.org/core/author | "Kong E.H."xsd:string |
| http://purl.uniprot.org/citations/21107322 | http://purl.uniprot.org/core/author | "Morris E.P."xsd:string |
| http://purl.uniprot.org/citations/21107322 | http://purl.uniprot.org/core/author | "Schreiber A."xsd:string |
| http://purl.uniprot.org/citations/21107322 | http://purl.uniprot.org/core/author | "da Fonseca P.C."xsd:string |
| http://purl.uniprot.org/citations/21107322 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21107322 | http://purl.uniprot.org/core/name | "Nature"xsd:string |
| http://purl.uniprot.org/citations/21107322 | http://purl.uniprot.org/core/pages | "274-278"xsd:string |
| http://purl.uniprot.org/citations/21107322 | http://purl.uniprot.org/core/title | "Structures of APC/C(Cdh1) with substrates identify Cdh1 and Apc10 as the D-box co-receptor."xsd:string |
| http://purl.uniprot.org/citations/21107322 | http://purl.uniprot.org/core/volume | "470"xsd:string |
| http://purl.uniprot.org/citations/21107322 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/21107322 |
| http://purl.uniprot.org/citations/21107322 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/21107322 |
| http://purl.uniprot.org/uniprot/#_A0A0A0MS51-mappedCitation-21107322 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21107322 |
| http://purl.uniprot.org/uniprot/#_A0A0A0MT01-mappedCitation-21107322 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21107322 |
| http://purl.uniprot.org/uniprot/#_A0A384MEF1-mappedCitation-21107322 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21107322 |
| http://purl.uniprot.org/uniprot/#_B3KS49-mappedCitation-21107322 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21107322 |
| http://purl.uniprot.org/uniprot/#_B7Z4U6-mappedCitation-21107322 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21107322 |
| http://purl.uniprot.org/uniprot/#_B7Z6N2-mappedCitation-21107322 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21107322 |
| http://purl.uniprot.org/uniprot/#_B7Z992-mappedCitation-21107322 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21107322 |
| http://purl.uniprot.org/uniprot/#_B7Z9A0-mappedCitation-21107322 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21107322 |