| http://purl.uniprot.org/citations/21129149 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21129149 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21129149 | http://www.w3.org/2000/01/rdf-schema#comment | "In the pathogenic yeast Candida glabrata, multidrug resistance is associated with the overexpression of drug efflux pumps caused by gain-of-function mutations in the CgPDR1 gene. CgPdr1p transcription factor, which activates the expression of several drug efflux transporter genes, is considered to be a promising target for compounds sensitizing the multidrug-resistant yeast cells. Here, we describe a cell-based screening system for detecting the inhibitory activity of compounds interfering with the CgPdr1p function in a heterologous genetic background of the hypersensitive Saccharomyces cerevisiae mutant strain. The screening is based on the ability to abrogate the growth defect of cells suffering from the galactose-induced and CgPdr1p-driven overexpression of a dominant lethal pma1(D378N) allele placed under the control of the ScPDR5 promoter. The system allows rapid identification of multidrug resistance reversal agents inhibiting the CgPdr1p activity or loss-of-function Cgpdr1 mutations, and is amenable to high-throughput screening on solid or liquid media."xsd:string |
| http://purl.uniprot.org/citations/21129149 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1567-1364.2010.00702.x"xsd:string |
| http://purl.uniprot.org/citations/21129149 | http://purl.org/dc/terms/identifier | "doi:10.1111/j.1567-1364.2010.00702.x"xsd:string |
| http://purl.uniprot.org/citations/21129149 | http://purl.uniprot.org/core/author | "Dzugasova V."xsd:string |
| http://purl.uniprot.org/citations/21129149 | http://purl.uniprot.org/core/author | "Dzugasova V."xsd:string |
| http://purl.uniprot.org/citations/21129149 | http://purl.uniprot.org/core/author | "Subik J."xsd:string |
| http://purl.uniprot.org/citations/21129149 | http://purl.uniprot.org/core/author | "Subik J."xsd:string |
| http://purl.uniprot.org/citations/21129149 | http://purl.uniprot.org/core/author | "Batova M."xsd:string |
| http://purl.uniprot.org/citations/21129149 | http://purl.uniprot.org/core/author | "Batova M."xsd:string |
| http://purl.uniprot.org/citations/21129149 | http://purl.uniprot.org/core/author | "Bialkova A."xsd:string |
| http://purl.uniprot.org/citations/21129149 | http://purl.uniprot.org/core/author | "Bialkova A."xsd:string |
| http://purl.uniprot.org/citations/21129149 | http://purl.uniprot.org/core/author | "Goffa E."xsd:string |
| http://purl.uniprot.org/citations/21129149 | http://purl.uniprot.org/core/author | "Goffa E."xsd:string |
| http://purl.uniprot.org/citations/21129149 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21129149 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21129149 | http://purl.uniprot.org/core/name | "FEMS Yeast Res."xsd:string |
| http://purl.uniprot.org/citations/21129149 | http://purl.uniprot.org/core/name | "FEMS Yeast Res."xsd:string |
| http://purl.uniprot.org/citations/21129149 | http://purl.uniprot.org/core/pages | "155-159"xsd:string |
| http://purl.uniprot.org/citations/21129149 | http://purl.uniprot.org/core/pages | "155-159"xsd:string |
| http://purl.uniprot.org/citations/21129149 | http://purl.uniprot.org/core/title | "A yeast cell-based system for screening Candida glabrata multidrug resistance reversal agents and selection of loss-of-function pdr1 mutants."xsd:string |
| http://purl.uniprot.org/citations/21129149 | http://purl.uniprot.org/core/title | "A yeast cell-based system for screening Candida glabrata multidrug resistance reversal agents and selection of loss-of-function pdr1 mutants."xsd:string |
| http://purl.uniprot.org/citations/21129149 | http://purl.uniprot.org/core/volume | "11"xsd:string |
| http://purl.uniprot.org/citations/21129149 | http://purl.uniprot.org/core/volume | "11"xsd:string |