| http://purl.uniprot.org/citations/21146485 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21146485 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21146485 | http://www.w3.org/2000/01/rdf-schema#comment | "Cyclophilin-40 (CyP40) is part of the immunophilin family and is found in Hsp90-containing protein complexes. We were interested in identifying proteins that interact with CyP40. CyP40-interacting proteins in HeLa cells were identified using the tandem affinity purification approach. Adenovirus expressing human CyP40 protein (Ad-CyP40), fused with streptavidin and calmodulin binding peptides at the N terminus, was generated. Proteins were separated on a sodium dodecyl sulfate-polyacrylamide gel electrophoresis gel after tandem affinity purification. Here 10 silver-stained protein bands that were enriched in the Ad-CyP40-infected lysate and the corresponding regions in the control lysate were excised, digested by trypsin, and identified by tandem mass spectrometric analysis. Of 11 interacting proteins that were identified, 4 (RACK1, Ku70, RPS3, and NF45) were expressed in rabbit reticulocyte lysate, bacteria, and MCF-7 cells. We confirmed that these proteins interact with CyP40. We observed that RACK1 suppressed the cobalt chloride-induced, hypoxia response element-dependent luciferase activity in MCF-7 cells but not in MCF-7 stable cells expressing approximately 10% of the cellular CyP40 content. In addition, RACK1 reduced the HIF-1α protein accumulation after cobalt chloride treatment, which was not observed when the CyP40 content was down-regulated. Collectively, we conclude that reduction of the HIF-1 α protein by RACK1 is CyP40-mediated."xsd:string |
| http://purl.uniprot.org/citations/21146485 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.ab.2010.12.007"xsd:string |
| http://purl.uniprot.org/citations/21146485 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.ab.2010.12.007"xsd:string |
| http://purl.uniprot.org/citations/21146485 | http://purl.uniprot.org/core/author | "Wang Y."xsd:string |
| http://purl.uniprot.org/citations/21146485 | http://purl.uniprot.org/core/author | "Wang Y."xsd:string |
| http://purl.uniprot.org/citations/21146485 | http://purl.uniprot.org/core/author | "Xie J."xsd:string |
| http://purl.uniprot.org/citations/21146485 | http://purl.uniprot.org/core/author | "Xie J."xsd:string |
| http://purl.uniprot.org/citations/21146485 | http://purl.uniprot.org/core/author | "Bhattacharya P."xsd:string |
| http://purl.uniprot.org/citations/21146485 | http://purl.uniprot.org/core/author | "Bhattacharya P."xsd:string |
| http://purl.uniprot.org/citations/21146485 | http://purl.uniprot.org/core/author | "Chu F."xsd:string |
| http://purl.uniprot.org/citations/21146485 | http://purl.uniprot.org/core/author | "Chu F."xsd:string |
| http://purl.uniprot.org/citations/21146485 | http://purl.uniprot.org/core/author | "Park M.S."xsd:string |
| http://purl.uniprot.org/citations/21146485 | http://purl.uniprot.org/core/author | "Park M.S."xsd:string |
| http://purl.uniprot.org/citations/21146485 | http://purl.uniprot.org/core/author | "Chan W.K."xsd:string |
| http://purl.uniprot.org/citations/21146485 | http://purl.uniprot.org/core/author | "Chan W.K."xsd:string |
| http://purl.uniprot.org/citations/21146485 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21146485 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21146485 | http://purl.uniprot.org/core/name | "Anal. Biochem."xsd:string |
| http://purl.uniprot.org/citations/21146485 | http://purl.uniprot.org/core/name | "Anal. Biochem."xsd:string |
| http://purl.uniprot.org/citations/21146485 | http://purl.uniprot.org/core/pages | "257-265"xsd:string |
| http://purl.uniprot.org/citations/21146485 | http://purl.uniprot.org/core/pages | "257-265"xsd:string |
| http://purl.uniprot.org/citations/21146485 | http://purl.uniprot.org/core/title | "Identification of cyclophilin-40-interacting proteins reveals potential cellular function of cyclophilin-40."xsd:string |
| http://purl.uniprot.org/citations/21146485 | http://purl.uniprot.org/core/title | "Identification of cyclophilin-40-interacting proteins reveals potential cellular function of cyclophilin-40."xsd:string |