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http://purl.uniprot.org/citations/21159825http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/21159825http://www.w3.org/2000/01/rdf-schema#comment"

Objective

It was recently reported that CD101 surface expression discriminates potency among CD4+CD25+ FoxP3+ regulatory T cells in the mouse. We investigated whether CD101 may also have a role in the suppressor function of regulatory T cells in humans given that the latter population may affect the autoimmune response in patients with rheumatoid arthritis (RA).

Methods

Sorted T cells and monocyte/macrophage cell populations were analyzed by flow cyto metry using conjugated antibodies specific for cell-surface markers. T cell proliferation assays were conducted by [(3)H]thymidine incorporation and CD8(high) cytotoxicity measurements by Cyto-Scan-LDH cytotoxicity assays. ELISA were used to measure cytokines in cell culture supernatants and Western blotting was performed for profiling mitogen-activated protein (MAP) kinase activation using specific antiphospholipid antibodies.

Results

CD101 expression coincided with PMA-induced monocyte/leukocyte lineage differentiation. CD8(high)CD101-T cells exhibited greater cytotoxic activity than CD8(high)CD101+ T cells, while no difference was observed between CD4CD25(high)CD101+ and CD4CD25(high)CD101-Treg inhibitory activity through responder T cells. LPS-induced proinflammatory cytokine production and p38 MAP kinase activation were made possible by ligation of CD101 with an anti-CD101 antibody F(ab')(2) fragment.

Conclusion

These results suggested a modulatory/coregulatory function of CD101 in the human immune system, in contrast to murine models, in which CD101 surface expression discriminates potency among FoxP3+ regulatory T cells. Cytotoxic CD8(high)CD101+ T cells were markedly less cytotoxic than CD8(high) T cells negative for the CD101 antigen and were conspicuously downregulated in patients with RA, suggesting a possible role for CD101 expression and function in the control of certain manifestations of RA pathology."xsd:string
http://purl.uniprot.org/citations/21159825http://purl.org/dc/terms/identifier"doi:10.3899/jrheum.100676"xsd:string
http://purl.uniprot.org/citations/21159825http://purl.uniprot.org/core/author"Bensussan A."xsd:string
http://purl.uniprot.org/citations/21159825http://purl.uniprot.org/core/author"Boumsell L."xsd:string
http://purl.uniprot.org/citations/21159825http://purl.uniprot.org/core/author"Mancini A."xsd:string
http://purl.uniprot.org/citations/21159825http://purl.uniprot.org/core/author"Chevalier X."xsd:string
http://purl.uniprot.org/citations/21159825http://purl.uniprot.org/core/author"Di Battista J.A."xsd:string
http://purl.uniprot.org/citations/21159825http://purl.uniprot.org/core/author"Jovanovic D.V."xsd:string
http://purl.uniprot.org/citations/21159825http://purl.uniprot.org/core/date"2011"xsd:gYear
http://purl.uniprot.org/citations/21159825http://purl.uniprot.org/core/name"J Rheumatol"xsd:string
http://purl.uniprot.org/citations/21159825http://purl.uniprot.org/core/pages"419-428"xsd:string
http://purl.uniprot.org/citations/21159825http://purl.uniprot.org/core/title"CD101 expression and function in normal and rheumatoid arthritis-affected human T cells and monocytes/macrophages."xsd:string
http://purl.uniprot.org/citations/21159825http://purl.uniprot.org/core/volume"38"xsd:string
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