| http://purl.uniprot.org/citations/21170310 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21170310 | http://www.w3.org/2000/01/rdf-schema#comment | "Polycomb proteins are epigenetic regulators that localize to developmental loci in the early embryo where they mediate lineage-specific gene repression. In Drosophila, these repressors are recruited to sequence elements by DNA binding proteins associated with Polycomb repressive complex 2 (PRC2). However, the sequences that recruit PRC2 in mammalian cells have remained obscure. To address this, we integrated a series of engineered bacterial artificial chromosomes into embryonic stem (ES) cells and examined their chromatin. We found that a 44 kb region corresponding to the Zfpm2 locus initiates de novo recruitment of PRC2. We then pinpointed a CpG island within this locus as both necessary and sufficient for PRC2 recruitment. Based on this causal demonstration and prior genomic analyses, we hypothesized that large GC-rich elements depleted of activating transcription factor motifs mediate PRC2 recruitment in mammals. We validated this model in two ways. First, we showed that a constitutively active CpG island is able to recruit PRC2 after excision of a cluster of activating motifs. Second, we showed that two 1 kb sequence intervals from the Escherichia coli genome with GC-contents comparable to a mammalian CpG island are both capable of recruiting PRC2 when integrated into the ES cell genome. Our findings demonstrate a causal role for GC-rich sequences in PRC2 recruitment and implicate a specific subset of CpG islands depleted of activating motifs as instrumental for the initial localization of this key regulator in mammalian genomes."xsd:string |
| http://purl.uniprot.org/citations/21170310 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pgen.1001244"xsd:string |
| http://purl.uniprot.org/citations/21170310 | http://purl.uniprot.org/core/author | "Bernstein B.E."xsd:string |
| http://purl.uniprot.org/citations/21170310 | http://purl.uniprot.org/core/author | "Chi A.S."xsd:string |
| http://purl.uniprot.org/citations/21170310 | http://purl.uniprot.org/core/author | "Issac B."xsd:string |
| http://purl.uniprot.org/citations/21170310 | http://purl.uniprot.org/core/author | "Ku M."xsd:string |
| http://purl.uniprot.org/citations/21170310 | http://purl.uniprot.org/core/author | "Truong T."xsd:string |
| http://purl.uniprot.org/citations/21170310 | http://purl.uniprot.org/core/author | "Mendenhall E.M."xsd:string |
| http://purl.uniprot.org/citations/21170310 | http://purl.uniprot.org/core/author | "Koche R.P."xsd:string |
| http://purl.uniprot.org/citations/21170310 | http://purl.uniprot.org/core/author | "Zhou V.W."xsd:string |
| http://purl.uniprot.org/citations/21170310 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/21170310 | http://purl.uniprot.org/core/name | "PLoS Genet"xsd:string |
| http://purl.uniprot.org/citations/21170310 | http://purl.uniprot.org/core/pages | "e1001244"xsd:string |
| http://purl.uniprot.org/citations/21170310 | http://purl.uniprot.org/core/title | "GC-rich sequence elements recruit PRC2 in mammalian ES cells."xsd:string |
| http://purl.uniprot.org/citations/21170310 | http://purl.uniprot.org/core/volume | "6"xsd:string |
| http://purl.uniprot.org/citations/21170310 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/21170310 |
| http://purl.uniprot.org/citations/21170310 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/21170310 |
| http://purl.uniprot.org/uniprot/#_P0C5Y9-mappedCitation-21170310 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21170310 |
| http://purl.uniprot.org/uniprot/#_P57053-mappedCitation-21170310 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21170310 |
| http://purl.uniprot.org/uniprot/#_Q15022-mappedCitation-21170310 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21170310 |
| http://purl.uniprot.org/uniprot/#_O43189-mappedCitation-21170310 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21170310 |
| http://purl.uniprot.org/uniprot/#_P58876-mappedCitation-21170310 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21170310 |
| http://purl.uniprot.org/uniprot/#_P62805-mappedCitation-21170310 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21170310 |
| http://purl.uniprot.org/uniprot/#_P62807-mappedCitation-21170310 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21170310 |