| http://purl.uniprot.org/citations/21179574 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21179574 | http://www.w3.org/2000/01/rdf-schema#comment | "Enterohaemorrhagic E. coli (EHEC) O157:H7 is a primary food-borne bacterial pathogen capable of causing life-threatening human infections which poses a serious challenge to public health worldwide. Intimin, the bacterial outer-membrane protein, plays a key role in the initiating process of EHEC infection. This activity is dependent upon translocation of the intimin receptor (Tir), the intimin binding partner of the bacteria-encoded host cell surface protein. Intimin has attracted considerable attention due to its potential function as an antibacterial drug target. Here, we report the crystal structure of the Tir-binding domain of intimin (Int188) from E. coli O157:H7 at 2.8 Å resolution, together with a mutant (IntN916Y) at 2.6 Å. We also built the structural model of EHEC intimin-Tir complex and analyzed the key binding residues. It suggested that the binding pattern of intimin and Tir between EHEC and Enteropathogenic E. coli (EPEC) adopt a similar mode and they can complement with each other. Detailed structural comparison indicates that there are four major points of structural variations between EHEC and EPEC intimins: one in Domain I (Ig-like domain), the other three located in Domain II (C-type lectin-like domain). These variations result in different binding affinities. These findings provide structural insight into the binding pattern of intimin to Tir and the molecular mechanism of EHEC O157: H7."xsd:string |
| http://purl.uniprot.org/citations/21179574 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0015285"xsd:string |
| http://purl.uniprot.org/citations/21179574 | http://purl.uniprot.org/core/author | "Feng Y."xsd:string |
| http://purl.uniprot.org/citations/21179574 | http://purl.uniprot.org/core/author | "Gao G.F."xsd:string |
| http://purl.uniprot.org/citations/21179574 | http://purl.uniprot.org/core/author | "Gao F."xsd:string |
| http://purl.uniprot.org/citations/21179574 | http://purl.uniprot.org/core/author | "Mao X."xsd:string |
| http://purl.uniprot.org/citations/21179574 | http://purl.uniprot.org/core/author | "Ma Y."xsd:string |
| http://purl.uniprot.org/citations/21179574 | http://purl.uniprot.org/core/author | "Fan Z."xsd:string |
| http://purl.uniprot.org/citations/21179574 | http://purl.uniprot.org/core/author | "Wang G."xsd:string |
| http://purl.uniprot.org/citations/21179574 | http://purl.uniprot.org/core/author | "Yan J."xsd:string |
| http://purl.uniprot.org/citations/21179574 | http://purl.uniprot.org/core/author | "Zeng H."xsd:string |
| http://purl.uniprot.org/citations/21179574 | http://purl.uniprot.org/core/author | "Peng H."xsd:string |
| http://purl.uniprot.org/citations/21179574 | http://purl.uniprot.org/core/author | "Guo G."xsd:string |
| http://purl.uniprot.org/citations/21179574 | http://purl.uniprot.org/core/author | "Zou Q."xsd:string |
| http://purl.uniprot.org/citations/21179574 | http://purl.uniprot.org/core/author | "Yi Y."xsd:string |
| http://purl.uniprot.org/citations/21179574 | http://purl.uniprot.org/core/date | "2010"xsd:gYear |
| http://purl.uniprot.org/citations/21179574 | http://purl.uniprot.org/core/name | "PLoS One"xsd:string |
| http://purl.uniprot.org/citations/21179574 | http://purl.uniprot.org/core/pages | "e15285"xsd:string |
| http://purl.uniprot.org/citations/21179574 | http://purl.uniprot.org/core/title | "Crystal structure of EHEC intimin: insights into the complementarity between EPEC and EHEC."xsd:string |
| http://purl.uniprot.org/citations/21179574 | http://purl.uniprot.org/core/volume | "5"xsd:string |
| http://purl.uniprot.org/citations/21179574 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/21179574 |
| http://purl.uniprot.org/citations/21179574 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/21179574 |
| http://purl.uniprot.org/uniprot/#_P43261-mappedCitation-21179574 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21179574 |
| http://purl.uniprot.org/uniprot/P43261 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/21179574 |