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http://purl.uniprot.org/citations/21180156http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/21180156http://www.w3.org/2000/01/rdf-schema#comment"

Aim

To investigate the role of TNF-alpha in vascular endothelial cells injury mediated by freezing/thaw ing PMN.

Methods

Freezing/thawing cell model was founded using rat PMN isolated by dextran sedimentation technique and VEC cultured in vitro. The injury level of VEC was indicated by measuring activity of LDH in medium. The number of frozen/thawed PMN adhering to VEC was counted with Phagocytizing reactive dyes the degree of frozen/thawed PMN and VEC adhesion. Expression of LFA-1 on the surface of frozen/thawed PMN was analyzed with flow cytometry.

Results

TNF-alpha could obviously upregulate expression of LFA-1 on surfaced of frozen/thawed PMN. Upregulation of LFA-1 expression promoted adhesion of frozen/thawed PMN and normal VEC,and aggravated VEC injury. Monoclonal antibody against LFA-1 could partly block adhesion of frozen/thawed PMN and normal VEC,and attenuate VEC injury.

Conclusion

TNF-alpha can promote expression of LFA-1 on surface of frozen/thawed PMN adhering of frozen/thawed PMN to normal VEC and VEC injury increase, monoclonal antibody against LFA-1 could partly block PMN-VEC adhesion and attenuate VEC injury."xsd:string
http://purl.uniprot.org/citations/21180156http://purl.uniprot.org/core/author"Liu J.J."xsd:string
http://purl.uniprot.org/citations/21180156http://purl.uniprot.org/core/author"Jin L.Y."xsd:string
http://purl.uniprot.org/citations/21180156http://purl.uniprot.org/core/author"Yang Z.R."xsd:string
http://purl.uniprot.org/citations/21180156http://purl.uniprot.org/core/author"Yan P.H."xsd:string
http://purl.uniprot.org/citations/21180156http://purl.uniprot.org/core/date"2005"xsd:gYear
http://purl.uniprot.org/citations/21180156http://purl.uniprot.org/core/name"Zhongguo Ying Yong Sheng Li Xue Za Zhi"xsd:string
http://purl.uniprot.org/citations/21180156http://purl.uniprot.org/core/pages"393-396"xsd:string
http://purl.uniprot.org/citations/21180156http://purl.uniprot.org/core/title"[Role of TNF-alpha in vascular endothelial cells injury mediated by frozen/thawed PMN]."xsd:string
http://purl.uniprot.org/citations/21180156http://purl.uniprot.org/core/volume"21"xsd:string
http://purl.uniprot.org/citations/21180156http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/21180156
http://purl.uniprot.org/citations/21180156http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/21180156
http://purl.uniprot.org/uniprot/#_A0A9K3Y824-mappedCitation-21180156http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/21180156
http://purl.uniprot.org/uniprot/#_B2RYB8-mappedCitation-21180156http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/21180156
http://purl.uniprot.org/uniprot/#_Q4KLK5-mappedCitation-21180156http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/21180156
http://purl.uniprot.org/uniprot/Q4KLK5http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/21180156
http://purl.uniprot.org/uniprot/A0A9K3Y824http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/21180156
http://purl.uniprot.org/uniprot/B2RYB8http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/21180156