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| http://purl.uniprot.org/citations/21185344 | http://www.w3.org/2000/01/rdf-schema#comment | "We examined the molecular and functional characterization of choline uptake into human neuroblastoma cell lines (SH-SY5Y: non-cholinergic and LA-N-2: cholinergic neuroblastoma), and the association between choline transport and acetylcholine (ACh) synthesis in these cells. Choline uptake was saturable and mediated by a single transport system. Removal of Na(+) from the uptake buffer strongly enhanced choline uptake. Choline uptake was inhibited by the choline analogue hemicholinium-3 (HC-3) and various organic cations, and was significantly decreased by acidification of the extracellular medium. The increase in choline uptake under Na(+)-free conditions was inhibited by a Na(+)/H(+) exchanger (NHE) inhibitor. Real-time PCR revealed that choline transporter-like protein 1 (CTL1), NHE1 and NHE5 mRNA are mainly expressed. Western blot and immunocytochemical analysis indicated that CTL1 protein was expressed in plasma membrane. ChAT mRNA was expressed at a much higher level in LA-N-2 cells than in SH-SY5Y cells. The conversion of choline to ACh was confirmed in both cells, and was enhanced in Na(+)-free conditions. These findings suggest that CTL1 is functionally expressed in both SH-SY5Y and LA-N-2 cells and is responsible for choline uptake that relies on a directed H(+) gradient as a driving force, and this transport functions in co-operation with NHE1 and NHE5. Furthermore, choline uptake through CTL1 is associated with ACh synthesis in cholinergic neuroblastoma cells."xsd:string |
| http://purl.uniprot.org/citations/21185344 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.neuint.2010.12.011"xsd:string |
| http://purl.uniprot.org/citations/21185344 | http://purl.uniprot.org/core/author | "Yamada T."xsd:string |
| http://purl.uniprot.org/citations/21185344 | http://purl.uniprot.org/core/author | "Tajima H."xsd:string |
| http://purl.uniprot.org/citations/21185344 | http://purl.uniprot.org/core/author | "Matsumiya T."xsd:string |
| http://purl.uniprot.org/citations/21185344 | http://purl.uniprot.org/core/author | "Inazu M."xsd:string |
| http://purl.uniprot.org/citations/21185344 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21185344 | http://purl.uniprot.org/core/name | "Neurochem Int"xsd:string |
| http://purl.uniprot.org/citations/21185344 | http://purl.uniprot.org/core/pages | "354-365"xsd:string |
| http://purl.uniprot.org/citations/21185344 | http://purl.uniprot.org/core/title | "Functional expression of choline transporter-like protein 1 (CTL1) in human neuroblastoma cells and its link to acetylcholine synthesis."xsd:string |
| http://purl.uniprot.org/citations/21185344 | http://purl.uniprot.org/core/volume | "58"xsd:string |
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