| http://purl.uniprot.org/citations/21193407 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21193407 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21193407 | http://www.w3.org/2000/01/rdf-schema#comment | "The glycosylphosphatidylinositol (GPI)-anchored neutrophil-specific receptor NB1 (CD177) presents the autoantigen proteinase 3 (PR3) on the membrane of a neutrophil subset. PR3-ANCA-activated neutrophils participate in small-vessel vasculitis. Since NB1 lacks an intracellular domain, we characterized components of the NB1 signaling complex that are pivotal for neutrophil activation. PR3-ANCA resulted in degranulation and superoxide production in the mNB1(pos)/PR3(high) neutrophils, but not in the mNB1(neg)/PR3(low) subset, whereas MPO-ANCA and fMLP caused similar responses. The NB1 signaling complex that was precipitated from plasma membranes contained the transmembrane receptor Mac-1 (CD11b/CD18) as shown by MS/MS analysis and immunoblotting. NB1 co-precipitation was less for CD11a and not detectable for CD11c. NB1 showed direct protein-protein interactions with both CD11b and CD11a by surface plasmon resonance analysis (SPR). However, when these integrins were presented as heterodimeric transmembrane proteins on transfected cells, only CD11b/CD18 (Mac-1)-transfected cells adhered to immobilized NB1 protein. This adhesion was inhibited by mAb against NB1, CD11b, and CD18. NB1, PR3, and Mac-1 were located within lipid rafts. In addition, confocal microscopy showed the strongest NB1 co-localization with CD11b and CD18 on the neutrophil. Stimulation with NB1-activating mAb triggered degranulation and superoxide production in mNB1(pos)/mPR3(high) neutrophils, and this effect was reduced using blocking antibodies to CD11b. CD11b blockade also inhibited PR3-ANCA-induced neutrophil activation, even when β2-integrin ligand-dependent signals were omitted. We establish the pivotal role of the NB1-Mac-1 receptor interaction for PR3-ANCA-mediated neutrophil activation."xsd:string |
| http://purl.uniprot.org/citations/21193407 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m110.171256"xsd:string |
| http://purl.uniprot.org/citations/21193407 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m110.171256"xsd:string |
| http://purl.uniprot.org/citations/21193407 | http://purl.uniprot.org/core/author | "Sporbert A."xsd:string |
| http://purl.uniprot.org/citations/21193407 | http://purl.uniprot.org/core/author | "Sporbert A."xsd:string |
| http://purl.uniprot.org/citations/21193407 | http://purl.uniprot.org/core/author | "Dittmar G."xsd:string |
| http://purl.uniprot.org/citations/21193407 | http://purl.uniprot.org/core/author | "Dittmar G."xsd:string |
| http://purl.uniprot.org/citations/21193407 | http://purl.uniprot.org/core/author | "Bayat B."xsd:string |
| http://purl.uniprot.org/citations/21193407 | http://purl.uniprot.org/core/author | "Bayat B."xsd:string |
| http://purl.uniprot.org/citations/21193407 | http://purl.uniprot.org/core/author | "Jerke U."xsd:string |
| http://purl.uniprot.org/citations/21193407 | http://purl.uniprot.org/core/author | "Jerke U."xsd:string |
| http://purl.uniprot.org/citations/21193407 | http://purl.uniprot.org/core/author | "Kettritz R."xsd:string |
| http://purl.uniprot.org/citations/21193407 | http://purl.uniprot.org/core/author | "Kettritz R."xsd:string |
| http://purl.uniprot.org/citations/21193407 | http://purl.uniprot.org/core/author | "Luft F."xsd:string |
| http://purl.uniprot.org/citations/21193407 | http://purl.uniprot.org/core/author | "Luft F."xsd:string |
| http://purl.uniprot.org/citations/21193407 | http://purl.uniprot.org/core/author | "Rolle S."xsd:string |
| http://purl.uniprot.org/citations/21193407 | http://purl.uniprot.org/core/author | "Rolle S."xsd:string |
| http://purl.uniprot.org/citations/21193407 | http://purl.uniprot.org/core/author | "Santoso S."xsd:string |
| http://purl.uniprot.org/citations/21193407 | http://purl.uniprot.org/core/author | "Santoso S."xsd:string |
| http://purl.uniprot.org/citations/21193407 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21193407 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21193407 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |
| http://purl.uniprot.org/citations/21193407 | http://purl.uniprot.org/core/name | "J. Biol. Chem."xsd:string |