| http://purl.uniprot.org/citations/21206031 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21206031 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21206031 | http://www.w3.org/2000/01/rdf-schema#comment | "ESX-1-secreted protein regulator (EspR; Rv3849) is a key regulator in Mycobacterium tuberculosis that delivers bacterial proteins into the host cell during infection. EspR binds directly to the Rv3616c-Rv3614c promoter and activates transcription and secretes itself from the bacterial cell by the ESX-1 system. The three-dimensional structure of EspR will aid in understanding the mechanisms by which it binds to the Rv3616c-Rv3614c promoter and is involved in transcriptional activation. This study will significantly aid in the development of EspR-based therapeutics against M. tuberculosis. The full-length EspR gene from M. tuberculosis (H37Rv strain) was cloned and overexpressed as a soluble protein in Escherichia coli. The protein was purified by affinity chromatography using His-tagged protein followed by size-exclusion chromatography. EspR was crystallized using polyethylene glycol 3350 as precipitant. The crystals diffracted to 3.2 Å resolution using synchrotron radiation of wavelength 0.97625 Å. The crystal belonged to space group P3(1)21 and contained three monomers in the asymmetric unit. Native and heavy-atom-derivatized data sets were collected from EspR crystals for use in ab initio structure-solution techniques."xsd:string |
| http://purl.uniprot.org/citations/21206031 | http://purl.org/dc/terms/identifier | "doi:10.1107/s174430911004618x"xsd:string |
| http://purl.uniprot.org/citations/21206031 | http://purl.org/dc/terms/identifier | "doi:10.1107/s174430911004618x"xsd:string |
| http://purl.uniprot.org/citations/21206031 | http://purl.uniprot.org/core/author | "Saxena A.K."xsd:string |
| http://purl.uniprot.org/citations/21206031 | http://purl.uniprot.org/core/author | "Saxena A.K."xsd:string |
| http://purl.uniprot.org/citations/21206031 | http://purl.uniprot.org/core/author | "Gangwar S.P."xsd:string |
| http://purl.uniprot.org/citations/21206031 | http://purl.uniprot.org/core/author | "Gangwar S.P."xsd:string |
| http://purl.uniprot.org/citations/21206031 | http://purl.uniprot.org/core/author | "Meena S.R."xsd:string |
| http://purl.uniprot.org/citations/21206031 | http://purl.uniprot.org/core/author | "Meena S.R."xsd:string |
| http://purl.uniprot.org/citations/21206031 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21206031 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21206031 | http://purl.uniprot.org/core/name | "Acta Crystallogr. F"xsd:string |
| http://purl.uniprot.org/citations/21206031 | http://purl.uniprot.org/core/name | "Acta Crystallogr. F"xsd:string |
| http://purl.uniprot.org/citations/21206031 | http://purl.uniprot.org/core/pages | "83-86"xsd:string |
| http://purl.uniprot.org/citations/21206031 | http://purl.uniprot.org/core/pages | "83-86"xsd:string |
| http://purl.uniprot.org/citations/21206031 | http://purl.uniprot.org/core/title | "Cloning, purification, crystallization and preliminary X-ray analysis of ESX-1-secreted protein regulator (EspR) from Mycobacterium tuberculosis."xsd:string |
| http://purl.uniprot.org/citations/21206031 | http://purl.uniprot.org/core/title | "Cloning, purification, crystallization and preliminary X-ray analysis of ESX-1-secreted protein regulator (EspR) from Mycobacterium tuberculosis."xsd:string |
| http://purl.uniprot.org/citations/21206031 | http://purl.uniprot.org/core/volume | "67"xsd:string |
| http://purl.uniprot.org/citations/21206031 | http://purl.uniprot.org/core/volume | "67"xsd:string |
| http://purl.uniprot.org/citations/21206031 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/21206031 |
| http://purl.uniprot.org/citations/21206031 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/21206031 |
| http://purl.uniprot.org/citations/21206031 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/21206031 |
| http://purl.uniprot.org/citations/21206031 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/21206031 |