| http://purl.uniprot.org/citations/21206974 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21206974 | http://www.w3.org/2000/01/rdf-schema#comment | "DNA methylation status in the CpG sites of promoter regions in cancer-related genes, such as PTCH, has traditionally been investigated using either dye-terminator sequencing or methylation-specific PCR. We aimed to study the PTCH gene promoter methylation in gynecological cancers, with a method that gives a quantitative measure of the methylation status of the promoter region of the studied gene, and for this purpose, we designed novel Pyrosequencing-based assays. Bisulfite-treated genomic DNA (bsDNA) was amplified by standard PCR and applied to novel Pyrosequencing® assays, in order to measure the methylated fraction (%) at each CpG site of the PTCH gene promoter. We analyzed 22 squamous cell cervical cancer tissue specimens (11 with good and 11 with poor outcomes after radiotherapy) and 5 ovarian cancer tissue specimens matched with 5 normal ovarian tissue specimens. Six optimized PCR protocols which generated 8 Pyrosequencing assays covering 63 CpG sites in the promoter regions 1 and 2 as well as the previously unanalyzed promoter region 3 in the PTCH gene were developed. The 27 tumor tissue specimens and 5 normal tissues did not show any methylation within any of the 63 CpG sites. Our data suggest that methylation of the PTCH promoter is not a high-prevalence feature of squamous cell cervical cancer or ovarian cancer, but Pyrosequencing assays are a good method for studying promoter methylation."xsd:string |
| http://purl.uniprot.org/citations/21206974 | http://purl.org/dc/terms/identifier | "doi:10.3892/ijo.2011.895"xsd:string |
| http://purl.uniprot.org/citations/21206974 | http://purl.uniprot.org/core/author | "Nilsson T.K."xsd:string |
| http://purl.uniprot.org/citations/21206974 | http://purl.uniprot.org/core/author | "Sorbe B."xsd:string |
| http://purl.uniprot.org/citations/21206974 | http://purl.uniprot.org/core/author | "Levanat S."xsd:string |
| http://purl.uniprot.org/citations/21206974 | http://purl.uniprot.org/core/author | "Lof-Ohlin Z.M."xsd:string |
| http://purl.uniprot.org/citations/21206974 | http://purl.uniprot.org/core/author | "Sabol M."xsd:string |
| http://purl.uniprot.org/citations/21206974 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21206974 | http://purl.uniprot.org/core/name | "Int J Oncol"xsd:string |
| http://purl.uniprot.org/citations/21206974 | http://purl.uniprot.org/core/pages | "685-692"xsd:string |
| http://purl.uniprot.org/citations/21206974 | http://purl.uniprot.org/core/title | "Promoter methylation in the PTCH gene in cervical epithelial cancer and ovarian cancer tissue as studied by eight novel Pyrosequencing(R) assays."xsd:string |
| http://purl.uniprot.org/citations/21206974 | http://purl.uniprot.org/core/volume | "38"xsd:string |
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