| http://purl.uniprot.org/citations/21212267 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21212267 | http://www.w3.org/2000/01/rdf-schema#comment | "It is well documented that protein kinase A (PKA) acts as a negative regulator of M phase promoting factor (MPF) by phosphorylating cell division cycle 25 homolog B (Cdc25B) in mammals. However, the molecular mechanism remains unclear. In this study, we identified PKA phosphorylation sites in vitro by LC-MS/MS analysis, including Ser(149), Ser(229), and Ser(321) of Cdc25B, and explored the role of Ser(149) in G(2)/M transition of fertilized mouse eggs. The results showed that the overexpressed Cdc25B-S149A mutant initiated efficient MPF activation by direct dephosphorylation of Cdc2-Tyr(15), resulting in triggering mitosis prior to Cdc25B-WT. Conversely, overexpression of the phosphomimic Cdc25B-S149D mutant showed no significant difference in comparison with the control groups. Furthermore, we found that Cdc25B-Ser(149) was phosphorylated at G(1) and S phases, whereas dephosphorylated at G(2) and M phases, and the phosphorylation of Cdc25B-Ser(149) was modulated by PKA in vivo. In addition, we examined endogenous and exogenous Cdc25B, which were expressed mostly in the cytoplasm at the G(1) and S phases and translocated to the nucleus at the G(2) phase. Collectively, our findings provide evidence that Ser(149) may be another potential PKA phosphorylation target of Cdc25B in G(2)/M transition of fertilized mouse eggs and Cdc25B as a direct downstream substrate of PKA in mammals, which plays important roles in the regulation of early development of mouse embryos."xsd:string |
| http://purl.uniprot.org/citations/21212267 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m110.150524"xsd:string |
| http://purl.uniprot.org/citations/21212267 | http://purl.uniprot.org/core/author | "Cui C."xsd:string |
| http://purl.uniprot.org/citations/21212267 | http://purl.uniprot.org/core/author | "Liu C."xsd:string |
| http://purl.uniprot.org/citations/21212267 | http://purl.uniprot.org/core/author | "Meng J."xsd:string |
| http://purl.uniprot.org/citations/21212267 | http://purl.uniprot.org/core/author | "Sun X."xsd:string |
| http://purl.uniprot.org/citations/21212267 | http://purl.uniprot.org/core/author | "Wu D."xsd:string |
| http://purl.uniprot.org/citations/21212267 | http://purl.uniprot.org/core/author | "Yang F."xsd:string |
| http://purl.uniprot.org/citations/21212267 | http://purl.uniprot.org/core/author | "Xiao J."xsd:string |
| http://purl.uniprot.org/citations/21212267 | http://purl.uniprot.org/core/author | "Yu B."xsd:string |
| http://purl.uniprot.org/citations/21212267 | http://purl.uniprot.org/core/author | "Hou J."xsd:string |
| http://purl.uniprot.org/citations/21212267 | http://purl.uniprot.org/core/author | "Fan H."xsd:string |
| http://purl.uniprot.org/citations/21212267 | http://purl.uniprot.org/core/author | "Wang E."xsd:string |
| http://purl.uniprot.org/citations/21212267 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21212267 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/21212267 | http://purl.uniprot.org/core/pages | "10356-10366"xsd:string |
| http://purl.uniprot.org/citations/21212267 | http://purl.uniprot.org/core/title | "Ser149 is another potential PKA phosphorylation target of Cdc25B in G2/M transition of fertilized mouse eggs."xsd:string |
| http://purl.uniprot.org/citations/21212267 | http://purl.uniprot.org/core/volume | "286"xsd:string |
| http://purl.uniprot.org/citations/21212267 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/21212267 |
| http://purl.uniprot.org/citations/21212267 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/21212267 |
| http://purl.uniprot.org/uniprot/#_Q3U535-mappedCitation-21212267 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21212267 |
| http://purl.uniprot.org/uniprot/#_P30306-mappedCitation-21212267 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21212267 |
| http://purl.uniprot.org/uniprot/#_Q5EG47-mappedCitation-21212267 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21212267 |
| http://purl.uniprot.org/uniprot/#_Q3TUQ7-mappedCitation-21212267 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21212267 |