| http://purl.uniprot.org/citations/21228347 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21228347 | http://www.w3.org/2000/01/rdf-schema#comment | "The CD3ε cytoplasmic tail contains a conserved proline-rich sequence (PRS) that influences TCR-CD3 expression and signaling. Although the PRS can bind the SH3.1 domain of the cytosolic adapter Nck, whether the PRS is constitutively available for Nck binding or instead represents a cryptic motif that is exposed via conformational change upon TCR-CD3 engagement (CD3Δc) is currently unresolved. Furthermore, the extent to which a cis-acting CD3ε basic amino acid-rich stretch (BRS), with its unique phosphoinositide-binding capability, might impact PRS accessibility is not clear. In this study, we found that freshly harvested primary thymocytes expressed low to moderate basal levels of Nck-accessible PRS ("open-CD3"), although most TCR-CD3 complexes were inaccessible to Nck ("closed-CD3"). Ag presentation in vivo induced open-CD3, accounting for half of the basal level found in thymocytes from MHC(+) mice. Additional stimulation with either anti-CD3 Abs or peptide-MHC ligands further elevated open-CD3 above basal levels, consistent with a model wherein antigenic engagement induces maximum PRS exposure. We also found that the open-CD3 conformation induced by APCs outlasted the time of ligand occupancy, marking receptors that had been engaged. Finally, CD3ε BRS-phosphoinositide interactions played no role in either adoption of the initial closed-CD3 conformation or induction of open-CD3 by Ab stimulation. Thus, a basal level of open-CD3 is succeeded by a higher, induced level upon TCR-CD3 engagement, involving CD3Δc and prolonged accessibility of the CD3ε PRS to Nck."xsd:string |
| http://purl.uniprot.org/citations/21228347 | http://purl.org/dc/terms/identifier | "doi:10.4049/jimmunol.1003225"xsd:string |
| http://purl.uniprot.org/citations/21228347 | http://purl.uniprot.org/core/author | "de la Cruz J."xsd:string |
| http://purl.uniprot.org/citations/21228347 | http://purl.uniprot.org/core/author | "Gil D."xsd:string |
| http://purl.uniprot.org/citations/21228347 | http://purl.uniprot.org/core/author | "Luescher I.F."xsd:string |
| http://purl.uniprot.org/citations/21228347 | http://purl.uniprot.org/core/author | "van Oers N.S."xsd:string |
| http://purl.uniprot.org/citations/21228347 | http://purl.uniprot.org/core/author | "Kruger T."xsd:string |
| http://purl.uniprot.org/citations/21228347 | http://purl.uniprot.org/core/author | "Schrum A.G."xsd:string |
| http://purl.uniprot.org/citations/21228347 | http://purl.uniprot.org/core/author | "Parks C.A."xsd:string |
| http://purl.uniprot.org/citations/21228347 | http://purl.uniprot.org/core/author | "Silge R.L."xsd:string |
| http://purl.uniprot.org/citations/21228347 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21228347 | http://purl.uniprot.org/core/name | "J Immunol"xsd:string |
| http://purl.uniprot.org/citations/21228347 | http://purl.uniprot.org/core/pages | "2282-2290"xsd:string |
| http://purl.uniprot.org/citations/21228347 | http://purl.uniprot.org/core/title | "Basal and antigen-induced exposure of the proline-rich sequence in CD3epsilon."xsd:string |
| http://purl.uniprot.org/citations/21228347 | http://purl.uniprot.org/core/volume | "186"xsd:string |
| http://purl.uniprot.org/citations/21228347 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/21228347 |
| http://purl.uniprot.org/citations/21228347 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/21228347 |
| http://purl.uniprot.org/uniprot/#_A0A1D8M9B3-mappedCitation-21228347 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21228347 |
| http://purl.uniprot.org/uniprot/#_A0A494B9G5-mappedCitation-21228347 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21228347 |
| http://purl.uniprot.org/uniprot/#_A0A494B9U2-mappedCitation-21228347 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21228347 |
| http://purl.uniprot.org/uniprot/#_A6H6M1-mappedCitation-21228347 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21228347 |
| http://purl.uniprot.org/uniprot/#_B0LAX5-mappedCitation-21228347 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21228347 |
| http://purl.uniprot.org/uniprot/#_P06342-mappedCitation-21228347 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21228347 |
| http://purl.uniprot.org/uniprot/#_P06343-mappedCitation-21228347 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21228347 |