| http://purl.uniprot.org/citations/2127276 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2127276 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/2127276 | http://www.w3.org/2000/01/rdf-schema#comment | "We have previously reported the isolation of two forms of cytochrome P-450 (P-450) with omega-hydroxylase activities toward prostaglandin A (PGA) and fatty acids, designated as P-450ka-1 and P-450ka-2, from kidney cortex microsomes of rabbits treated with di(2-ethylhexyl)phthalate [Kusunose, E. et al. (1989) J. Biochem. 106, 194-196]. In the present work, we have purified and characterized two additional forms of rabbit kidney fatty acid omega-hydroxylase, designated as P-450kc and P-450kd. The purified P-450kc and P-450kd had specific contents of 13 and 16 nmol of P-450/mg of protein, with apparent molecular weights of 52,000 and 55,000 on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), respectively. Both the forms showed absorption maxima at 450 nm in the carbon monoxide-difference spectra for their reduced forms. These P-450s efficiently catalyzed the omega- and (omega-1)-hydroxylation of fatty acids such as caprate, laurate, myristate, and palmitate, in a reconstituted system containing P-450, NADPH-P-450 reductase, and phosphatidylcholine. Cytochrome b5 stimulated the reactions to only a slight extent. They had no detectable activity toward PGA and several xenobiotics tested. The two P-450s showed different peptide map patterns after limited proteolysis with papain or Staphylococcus aureus V8 protease.(ABSTRACT TRUNCATED AT 250 WORDS)"xsd:string |
| http://purl.uniprot.org/citations/2127276 | http://purl.org/dc/terms/identifier | "doi:10.1093/oxfordjournals.jbchem.a123239"xsd:string |
| http://purl.uniprot.org/citations/2127276 | http://purl.org/dc/terms/identifier | "doi:10.1093/oxfordjournals.jbchem.a123239"xsd:string |
| http://purl.uniprot.org/citations/2127276 | http://purl.uniprot.org/core/author | "Yamamoto S."xsd:string |
| http://purl.uniprot.org/citations/2127276 | http://purl.uniprot.org/core/author | "Yamamoto S."xsd:string |
| http://purl.uniprot.org/citations/2127276 | http://purl.uniprot.org/core/author | "Kubota I."xsd:string |
| http://purl.uniprot.org/citations/2127276 | http://purl.uniprot.org/core/author | "Kubota I."xsd:string |
| http://purl.uniprot.org/citations/2127276 | http://purl.uniprot.org/core/author | "Yokotani N."xsd:string |
| http://purl.uniprot.org/citations/2127276 | http://purl.uniprot.org/core/author | "Yokotani N."xsd:string |
| http://purl.uniprot.org/citations/2127276 | http://purl.uniprot.org/core/author | "Kusunose E."xsd:string |
| http://purl.uniprot.org/citations/2127276 | http://purl.uniprot.org/core/author | "Kusunose E."xsd:string |
| http://purl.uniprot.org/citations/2127276 | http://purl.uniprot.org/core/author | "Kusunose M."xsd:string |
| http://purl.uniprot.org/citations/2127276 | http://purl.uniprot.org/core/author | "Kusunose M."xsd:string |
| http://purl.uniprot.org/citations/2127276 | http://purl.uniprot.org/core/author | "Yoshimura R."xsd:string |
| http://purl.uniprot.org/citations/2127276 | http://purl.uniprot.org/core/author | "Yoshimura R."xsd:string |
| http://purl.uniprot.org/citations/2127276 | http://purl.uniprot.org/core/date | "1990"xsd:gYear |
| http://purl.uniprot.org/citations/2127276 | http://purl.uniprot.org/core/date | "1990"xsd:gYear |
| http://purl.uniprot.org/citations/2127276 | http://purl.uniprot.org/core/name | "J. Biochem."xsd:string |
| http://purl.uniprot.org/citations/2127276 | http://purl.uniprot.org/core/name | "J. Biochem."xsd:string |
| http://purl.uniprot.org/citations/2127276 | http://purl.uniprot.org/core/pages | "544-548"xsd:string |
| http://purl.uniprot.org/citations/2127276 | http://purl.uniprot.org/core/pages | "544-548"xsd:string |
| http://purl.uniprot.org/citations/2127276 | http://purl.uniprot.org/core/title | "Purification and characterization of two forms of fatty acid omega-hydroxylase cytochrome P-450 from rabbit kidney cortex microsomes."xsd:string |
| http://purl.uniprot.org/citations/2127276 | http://purl.uniprot.org/core/title | "Purification and characterization of two forms of fatty acid omega-hydroxylase cytochrome P-450 from rabbit kidney cortex microsomes."xsd:string |