| http://purl.uniprot.org/citations/21278227 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21278227 | http://www.w3.org/2000/01/rdf-schema#comment | "We have tested the usefulness of several commercial anti-CD33 monoclonal antibodies (mAb) to determine the expression and localization of the two CD33 isoforms on several hematopoietic cell lines. The expression of the isoform CD33m, a CD33 transmembrane splice variant lacking the ligand-binding V immunoglobulin (Ig)-like domain, was detected by RT-polymerase chain reaction, western blot, confocal microscopy and flow cytometry on the membrane of several human cell types. CD33m was only detected by the anti-CD33 mAb HIM3-4 on the cell surface, whereas WM53, P67.6, 4D3, HIM3-4, WM54, D3HL60.251 or MY9 detected the CD33M isoform, indicating that HIM3-4 is the only mAb recognizing CD33 C(2) Ig domain. Accordingly, HIM3-4 binding to CD33 did not interfere with the binding of other antibodies against the CD33 V-domain. P67.6 mAb interfered with recognition by the rest of antibodies specific for the V domain. HIM3-4 staining could be increased after the sialidase treatment of all CD33(+) cells. However, this increase was stronger in activated T cells, suggesting a CD33 masking state in this cell population. Confocal microscopy analysis of CD33m HEK 293T-transfected cells revealed that this protein is expressed on the cell membrane and also detected in the Golgi compartment. CD33 is constitutively located outside the lipid raft domains, whereas cross-linked CD33 is highly recruited to this signaling platform. The unique ability of HIM3-4 mAb to detect the masking state of CD33 on different cell lineages makes it a good tool to improve the knowledge of the biological role of this sialic acid-binding Ig-like lectin."xsd:string |
| http://purl.uniprot.org/citations/21278227 | http://purl.org/dc/terms/identifier | "doi:10.1093/glycob/cwq220"xsd:string |
| http://purl.uniprot.org/citations/21278227 | http://purl.uniprot.org/core/author | "Martinez-Esparza M."xsd:string |
| http://purl.uniprot.org/citations/21278227 | http://purl.uniprot.org/core/author | "Perez-Oliva A.B."xsd:string |
| http://purl.uniprot.org/citations/21278227 | http://purl.uniprot.org/core/author | "Garcia-Penarrubia P."xsd:string |
| http://purl.uniprot.org/citations/21278227 | http://purl.uniprot.org/core/author | "Hernandez-Caselles T."xsd:string |
| http://purl.uniprot.org/citations/21278227 | http://purl.uniprot.org/core/author | "Corral-San Miguel R."xsd:string |
| http://purl.uniprot.org/citations/21278227 | http://purl.uniprot.org/core/author | "Vicente-Fernandez J.J."xsd:string |
| http://purl.uniprot.org/citations/21278227 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21278227 | http://purl.uniprot.org/core/name | "Glycobiology"xsd:string |
| http://purl.uniprot.org/citations/21278227 | http://purl.uniprot.org/core/pages | "757-770"xsd:string |
| http://purl.uniprot.org/citations/21278227 | http://purl.uniprot.org/core/title | "Epitope mapping, expression and post-translational modifications of two isoforms of CD33 (CD33M and CD33m) on lymphoid and myeloid human cells."xsd:string |
| http://purl.uniprot.org/citations/21278227 | http://purl.uniprot.org/core/volume | "21"xsd:string |
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