| http://purl.uniprot.org/citations/21280220 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21280220 | http://www.w3.org/2000/01/rdf-schema#comment | "Topoisomerase (topo) II catalyzes topological changes in DNA. Although both human isozymes, topo IIα and β are phosphorylated, site-specific phosphorylation of topo IIβ is poorly characterized. Using LC-MS/MS analysis of topo IIβ, cleaved with trypsin, Arg C or cyanogen bromide (CNBr) plus trypsin, we detected four +80-Da modified sites: tyr656, ser1395, thr1426 and ser1545. Phosphorylation at ser1395, thr1426 and ser1545 was established based on neutral loss of H(3) PO(4) (-98 Da) in the CID spectra and on differences in 2-D-phosphopeptide maps of (32) P-labeled wild-type (WT) and S1395A or T1426A/S1545A mutant topo IIβ. However, phosphorylation at tyr656 could not be verified by 2-D-phosphopeptide mapping of (32) P-labeled WT and Y656F mutant protein or by Western blotting with phosphotyrosine-specific antibodies. Since the +80-Da modification on tyr656 was observed exclusively during cleavage with CNBr and trypsin, this modification likely represented bromination, which occurred during CNBr cleavage. Re-evaluation of the CID spectra identified +78/+80-Da fragment ions in CID spectra of two peptides containing tyr656 and tyr711, confirming bromination. Interestingly, mutation of only tyr656, but not ser1395, thr1326 or ser1545, decreased topo IIβ activity, suggesting a functional role for tyr656. These results, while identifying an important tyrosine in topo IIβ, underscore the importance of careful interpretation of modifications having the same nominal mass."xsd:string |
| http://purl.uniprot.org/citations/21280220 | http://purl.org/dc/terms/identifier | "doi:10.1002/pmic.201000194"xsd:string |
| http://purl.uniprot.org/citations/21280220 | http://purl.uniprot.org/core/author | "Kinter M."xsd:string |
| http://purl.uniprot.org/citations/21280220 | http://purl.uniprot.org/core/author | "Ganapathi M.K."xsd:string |
| http://purl.uniprot.org/citations/21280220 | http://purl.uniprot.org/core/author | "Chikamori K."xsd:string |
| http://purl.uniprot.org/citations/21280220 | http://purl.uniprot.org/core/author | "Ganapathi R."xsd:string |
| http://purl.uniprot.org/citations/21280220 | http://purl.uniprot.org/core/author | "Grozav A.G."xsd:string |
| http://purl.uniprot.org/citations/21280220 | http://purl.uniprot.org/core/author | "Kozuki T."xsd:string |
| http://purl.uniprot.org/citations/21280220 | http://purl.uniprot.org/core/author | "Petrescu A.J."xsd:string |
| http://purl.uniprot.org/citations/21280220 | http://purl.uniprot.org/core/author | "Willard B.B."xsd:string |
| http://purl.uniprot.org/citations/21280220 | http://purl.uniprot.org/core/author | "Micluta M.A."xsd:string |
| http://purl.uniprot.org/citations/21280220 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21280220 | http://purl.uniprot.org/core/name | "Proteomics"xsd:string |
| http://purl.uniprot.org/citations/21280220 | http://purl.uniprot.org/core/pages | "829-842"xsd:string |
| http://purl.uniprot.org/citations/21280220 | http://purl.uniprot.org/core/title | "Tyrosine 656 in topoisomerase IIbeta is important for the catalytic activity of the enzyme: Identification based on artifactual +80-Da modification at this site."xsd:string |
| http://purl.uniprot.org/citations/21280220 | http://purl.uniprot.org/core/volume | "11"xsd:string |
| http://purl.uniprot.org/citations/21280220 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/21280220 |
| http://purl.uniprot.org/citations/21280220 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/21280220 |
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| http://purl.uniprot.org/uniprot/#_B7ZB27-mappedCitation-21280220 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21280220 |
| http://purl.uniprot.org/uniprot/#_L8E8K5-mappedCitation-21280220 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21280220 |
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| http://purl.uniprot.org/uniprot/#_Q59H80-mappedCitation-21280220 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21280220 |