| http://purl.uniprot.org/citations/21297979 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21297979 | http://www.w3.org/2000/01/rdf-schema#comment | "BackgroundHighly Expressed in Cancer protein 1 (Hec1) is a constituent of the Ndc80 complex, a kinetochore component that has been shown to have a fundamental role in stable kinetochore-microtubule attachment, chromosome alignment and spindle checkpoint activation at mitosis. HEC1 RNA is found up-regulated in several cancer cells, suggesting a role for HEC1 deregulation in cancer. In light of this, we have investigated the consequences of experimentally-driven Hec1 expression on mitosis and chromosome segregation in an inducible expression system from human cells.Methodology/principal findingsOverexpression of Hec1 could never be obtained in HeLa clones inducibly expressing C-terminally tagged Hec1 or untagged Hec1, suggesting that Hec1 cellular levels are tightly controlled. On the contrary, a chimeric protein with an EGFP tag fused to the Hec1 N-terminus accumulated in cells and disrupted mitotic division. EGFP-Hec1 cells underwent altered chromosome segregation within multipolar spindles that originated from centriole splitting. We found that EGFP-Hec1 assembled a mutant Ndc80 complex that was unable to rescue the mitotic phenotypes of Hec1 depletion. Kinetochores harboring EGFP-Hec1 formed persisting lateral microtubule-kinetochore interactions that recruited the plus-end depolymerase MCAK and the microtubule stabilizing protein HURP on K-fibers. In these conditions the plus-end kinesin CENP-E was preferentially retained at kinetochores. RNAi-mediated CENP-E depletion further demonstrated that CENP-E function was required for multipolar spindle formation in EGFP-Hec1 expressing cells.Conclusions/significanceOur study suggests that modifications on Hec1 N-terminal tail can alter kinetochore-microtubule attachment stability and influence Ndc80 complex function independently from the intracellular levels of the protein. N-terminally modified Hec1 promotes spindle pole fragmentation by CENP-E-mediated plus-end directed kinetochore pulling forces that disrupt the fine balance of kinetochore- and centrosome-associated forces regulating spindle bipolarity. Overall, our findings support a model in which centrosome integrity is influenced by the pathways regulating kinetochore-microtubule attachment stability."xsd:string |
| http://purl.uniprot.org/citations/21297979 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0016307"xsd:string |
| http://purl.uniprot.org/citations/21297979 | http://purl.uniprot.org/core/author | "Musacchio A."xsd:string |
| http://purl.uniprot.org/citations/21297979 | http://purl.uniprot.org/core/author | "Ciferri C."xsd:string |
| http://purl.uniprot.org/citations/21297979 | http://purl.uniprot.org/core/author | "Fiore M."xsd:string |
| http://purl.uniprot.org/citations/21297979 | http://purl.uniprot.org/core/author | "Mattiuzzo M."xsd:string |
| http://purl.uniprot.org/citations/21297979 | http://purl.uniprot.org/core/author | "Vargiu G."xsd:string |
| http://purl.uniprot.org/citations/21297979 | http://purl.uniprot.org/core/author | "Degrassi F."xsd:string |
| http://purl.uniprot.org/citations/21297979 | http://purl.uniprot.org/core/author | "Totta P."xsd:string |
| http://purl.uniprot.org/citations/21297979 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21297979 | http://purl.uniprot.org/core/name | "PLoS One"xsd:string |
| http://purl.uniprot.org/citations/21297979 | http://purl.uniprot.org/core/pages | "e16307"xsd:string |
| http://purl.uniprot.org/citations/21297979 | http://purl.uniprot.org/core/title | "Abnormal kinetochore-generated pulling forces from expressing a N-terminally modified Hec1."xsd:string |
| http://purl.uniprot.org/citations/21297979 | http://purl.uniprot.org/core/volume | "6"xsd:string |
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