| http://purl.uniprot.org/citations/21304964 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21304964 | http://www.w3.org/2000/01/rdf-schema#comment | "Recent studies demonstrated expression and activity of the intracellular cortisone-cortisol shuttle 11beta-hydroxysteroid dehydrogenase type 1 (11beta-HSD1) in skeletal muscle and inhibition of 11beta-HSD1 in muscle cells improved insulin sensitivity. Glucocorticoids induce muscle atrophy via increased expression of the E3 ubiquitin ligases Atrogin-1 (Muscle Atrophy F-box (MAFbx)) and MuRF-1 (Muscle RING-Finger-1). We hypothesized that 11beta-HSD1 controls glucocorticoid-induced expression of atrophy E3 ubiquitin ligases in skeletal muscle. Primary human myoblasts were generated from healthy volunteers. 11beta-HSD1-dependent protein degradation was analyzed by [(3)H]-tyrosine release assay. RT-PCR was used to determine mRNA expression of E3 ubiquitin ligases and 11beta-HSD1 activity was measured by conversion of radioactively labeled [(3)H]-cortisone to [(3)H]-cortisol separated by thin-layer chromatography. We here demonstrate that 11beta-HSD1 is expressed and biologically active in interconverting cortisone to active cortisol in murine skeletal muscle cells (C2C12) as well as in primary human myotubes. 11Beta-HSD1 expression increased during differentiation from myoblasts to mature myotubes (p < 0.01), suggesting a role of 11beta-HSD1 in skeletal muscle growth and differentiation. Treatment with cortisone increased protein degradation by about 20% (p < 0.001), which was paralleled by an elevation of Atrogin-1 and MuRF-1 mRNA expression (p < 0.01, respectively). Notably, pre-treatment with the 11beta-HSD1 inhibitor carbenoxolone (Cbx) completely abolished the effect of cortisone on protein degradation as well as on Atrogin-1 and MuRF-1 expression. In summary, our data suggest that 11beta-HSD1 controls glucocorticoid-induced protein degradation in human and murine skeletal muscle via regulation of the E3 ubiquitin ligases Atrogin-1 and MuRF-1."xsd:string |
| http://purl.uniprot.org/citations/21304964 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0016674"xsd:string |
| http://purl.uniprot.org/citations/21304964 | http://purl.uniprot.org/core/author | "Adams S."xsd:string |
| http://purl.uniprot.org/citations/21304964 | http://purl.uniprot.org/core/author | "Mai K."xsd:string |
| http://purl.uniprot.org/citations/21304964 | http://purl.uniprot.org/core/author | "Spranger J."xsd:string |
| http://purl.uniprot.org/citations/21304964 | http://purl.uniprot.org/core/author | "Spuler S."xsd:string |
| http://purl.uniprot.org/citations/21304964 | http://purl.uniprot.org/core/author | "Andres J."xsd:string |
| http://purl.uniprot.org/citations/21304964 | http://purl.uniprot.org/core/author | "Fielitz J."xsd:string |
| http://purl.uniprot.org/citations/21304964 | http://purl.uniprot.org/core/author | "Biedasek K."xsd:string |
| http://purl.uniprot.org/citations/21304964 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21304964 | http://purl.uniprot.org/core/name | "PLoS One"xsd:string |
| http://purl.uniprot.org/citations/21304964 | http://purl.uniprot.org/core/pages | "e16674"xsd:string |
| http://purl.uniprot.org/citations/21304964 | http://purl.uniprot.org/core/title | "Skeletal muscle 11beta-HSD1 controls glucocorticoid-induced proteolysis and expression of E3 ubiquitin ligases atrogin-1 and MuRF-1."xsd:string |
| http://purl.uniprot.org/citations/21304964 | http://purl.uniprot.org/core/volume | "6"xsd:string |
| http://purl.uniprot.org/citations/21304964 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/21304964 |
| http://purl.uniprot.org/citations/21304964 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/21304964 |
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| http://purl.uniprot.org/uniprot/#_Q3TJI8-mappedCitation-21304964 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21304964 |