| http://purl.uniprot.org/citations/21317292 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21317292 | http://www.w3.org/2000/01/rdf-schema#comment | "Directed evolution is a valuable technique to improve enzyme activity in the absence of a priori structural knowledge, which can be typically enhanced via structure-guided strategies. In this study, a combination of both whole-gene error-prone polymerase chain reaction and site-saturation mutagenesis enabled the rapid identification of mutations that improved RmlA activity toward non-native substrates. These mutations have been shown to improve activities over 10-fold for several targeted substrates, including non-native pyrimidine- and purine-based NTPs as well as non-native D- and L-sugars (both α- and β-isomers). This study highlights the first broadly applicable high throughput sugar-1-phosphate nucleotidyltransferase screen and the first proof of concept for the directed evolution of this enzyme class toward the identification of uniquely permissive RmlA variants."xsd:string |
| http://purl.uniprot.org/citations/21317292 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m110.206433"xsd:string |
| http://purl.uniprot.org/citations/21317292 | http://purl.uniprot.org/core/author | "Bingman C.A."xsd:string |
| http://purl.uniprot.org/citations/21317292 | http://purl.uniprot.org/core/author | "Phillips G.N. Jr."xsd:string |
| http://purl.uniprot.org/citations/21317292 | http://purl.uniprot.org/core/author | "Chang A."xsd:string |
| http://purl.uniprot.org/citations/21317292 | http://purl.uniprot.org/core/author | "Thorson J.S."xsd:string |
| http://purl.uniprot.org/citations/21317292 | http://purl.uniprot.org/core/author | "Moretti R."xsd:string |
| http://purl.uniprot.org/citations/21317292 | http://purl.uniprot.org/core/author | "Peltier-Pain P."xsd:string |
| http://purl.uniprot.org/citations/21317292 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21317292 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/21317292 | http://purl.uniprot.org/core/pages | "13235-13243"xsd:string |
| http://purl.uniprot.org/citations/21317292 | http://purl.uniprot.org/core/title | "Expanding the nucleotide and sugar 1-phosphate promiscuity of nucleotidyltransferase RmlA via directed evolution."xsd:string |
| http://purl.uniprot.org/citations/21317292 | http://purl.uniprot.org/core/volume | "286"xsd:string |
| http://purl.uniprot.org/citations/21317292 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/21317292 |
| http://purl.uniprot.org/citations/21317292 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/21317292 |
| http://purl.uniprot.org/uniprot/#_P26393-mappedCitation-21317292 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21317292 |
| http://purl.uniprot.org/uniprot/P26393 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/21317292 |