| http://purl.uniprot.org/citations/21329668 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21329668 | http://www.w3.org/2000/01/rdf-schema#comment | "Natural cytotoxicity receptor 1 (NCR1, NKp46) binds to heparin and heparan sulfate; however, other natural ligands for NKp46 have yet to be elucidated. Using the recombinant extracellular region (coding for AA 22-258) of NKp46 tagged with 6× His (NKp46-H6), and mutants K136Q, R139Q, H142Q, R145Q, and K149Q, we determined their binding affinities to sulfate- and NeuAc-containing glycans-coated plates. NKp46-H6 directly bound to plates coated with heparin- and heparan sulfate-conjugated bovine serum albumin with K(d) values of 770 and 850 nM, respectively. The binding of NKp46-H6 to heparin-BSA was suppressed by soluble heparin, herparan sulfate, fucoidan, λ-carrageenan, and dextran sulfate, but not by 2-O-, 6-O-, and N-desulfated heparin. NKp46-H6 also bound to multimeric sialyl Lewis X expressing transferrin secreted by human hepatoma HepG2 cells (HepTF) with a K(d) value of 530 nM, but not to desialylated HepTF, commercially available TF, or 1-acid glycoprotein. Moreover, mutants R139Q, R145Q, and K149Q had significantly reduced binding to these sulfate-containing glycans, and K136Q and K149Q to HepTF, indicating that NKp46 binds to sulfate- and 2,3-NeuAc-containing glycans mainly via ionic interactions. However, the binding sites of NKp46 were different."xsd:string |
| http://purl.uniprot.org/citations/21329668 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.bbrc.2011.02.050"xsd:string |
| http://purl.uniprot.org/citations/21329668 | http://purl.uniprot.org/core/author | "Ito K."xsd:string |
| http://purl.uniprot.org/citations/21329668 | http://purl.uniprot.org/core/author | "Matsumoto K."xsd:string |
| http://purl.uniprot.org/citations/21329668 | http://purl.uniprot.org/core/author | "Azuma Y."xsd:string |
| http://purl.uniprot.org/citations/21329668 | http://purl.uniprot.org/core/author | "Sakurai M."xsd:string |
| http://purl.uniprot.org/citations/21329668 | http://purl.uniprot.org/core/author | "Yanai K."xsd:string |
| http://purl.uniprot.org/citations/21329668 | http://purl.uniprot.org/core/author | "Higai K."xsd:string |
| http://purl.uniprot.org/citations/21329668 | http://purl.uniprot.org/core/author | "Shinoda C."xsd:string |
| http://purl.uniprot.org/citations/21329668 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21329668 | http://purl.uniprot.org/core/name | "Biochem Biophys Res Commun"xsd:string |
| http://purl.uniprot.org/citations/21329668 | http://purl.uniprot.org/core/pages | "377-382"xsd:string |
| http://purl.uniprot.org/citations/21329668 | http://purl.uniprot.org/core/title | "Binding of natural cytotoxicity receptor NKp46 to sulfate- and alpha2,3-NeuAc-containing glycans and its mutagenesis."xsd:string |
| http://purl.uniprot.org/citations/21329668 | http://purl.uniprot.org/core/volume | "406"xsd:string |
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