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http://purl.uniprot.org/citations/21343311http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/21343311http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/21343311http://www.w3.org/2000/01/rdf-schema#comment"The activities of transcription factors (TFs) require interactions with specific DNA sequences and other regulatory proteins. To detect such interactions in Arabidopsis, we developed a high-throughput screening system with a Gateway-compatible Gal4-AD-TF library of 1589 Arabidopsis TFs, which can be easily screened by mating-based yeast-one-hybrid (Y1H) and yeast-two-hybrid (Y2H) methods. The efficiency of the system was validated by examining two well-characterized TF-DNA and TF-protein interactions: the CHE-CCA1 promoter interaction by Y1H and NPR1-TGAs interactions by Y2H. We used this system to identify eight TFs that interact with a Mediator subunit, Med25, a key regulator in JA signaling. We identified five TFs that interacted with the GCC-box cis-element in the promoter of PDF1.2, a downstream gene of Med25. We found that three of these TFs, all from the AP2-EREBP family, interact directly both with Med25 and the GCC-box of PDF1.2, suggesting that Med25 regulates PDF1.2 expression through these three TFs. These results demonstrate that this high-throughput Y1H/Y2H screening system is an efficient tool for studying transcriptional regulation networks in Arabidopsis. This system will be available for other Arabidopsis researchers, and thus it provides a vital resource for the Arabidopsis community."xsd:string
http://purl.uniprot.org/citations/21343311http://purl.org/dc/terms/identifier"doi:10.1093/mp/ssr002"xsd:string
http://purl.uniprot.org/citations/21343311http://purl.org/dc/terms/identifier"doi:10.1093/mp/ssr002"xsd:string
http://purl.uniprot.org/citations/21343311http://purl.uniprot.org/core/author"Kondou Y."xsd:string
http://purl.uniprot.org/citations/21343311http://purl.uniprot.org/core/author"Kondou Y."xsd:string
http://purl.uniprot.org/citations/21343311http://purl.uniprot.org/core/author"Matsui M."xsd:string
http://purl.uniprot.org/citations/21343311http://purl.uniprot.org/core/author"Matsui M."xsd:string
http://purl.uniprot.org/citations/21343311http://purl.uniprot.org/core/author"Zhang L."xsd:string
http://purl.uniprot.org/citations/21343311http://purl.uniprot.org/core/author"Zhang L."xsd:string
http://purl.uniprot.org/citations/21343311http://purl.uniprot.org/core/author"Yang Y."xsd:string
http://purl.uniprot.org/citations/21343311http://purl.uniprot.org/core/author"Yang Y."xsd:string
http://purl.uniprot.org/citations/21343311http://purl.uniprot.org/core/author"Gu T."xsd:string
http://purl.uniprot.org/citations/21343311http://purl.uniprot.org/core/author"Gu T."xsd:string
http://purl.uniprot.org/citations/21343311http://purl.uniprot.org/core/author"Qu L.J."xsd:string
http://purl.uniprot.org/citations/21343311http://purl.uniprot.org/core/author"Qu L.J."xsd:string
http://purl.uniprot.org/citations/21343311http://purl.uniprot.org/core/author"Miao J."xsd:string
http://purl.uniprot.org/citations/21343311http://purl.uniprot.org/core/author"Miao J."xsd:string
http://purl.uniprot.org/citations/21343311http://purl.uniprot.org/core/author"Gu H.Y."xsd:string
http://purl.uniprot.org/citations/21343311http://purl.uniprot.org/core/author"Gu H.Y."xsd:string
http://purl.uniprot.org/citations/21343311http://purl.uniprot.org/core/author"Liu S.N."xsd:string
http://purl.uniprot.org/citations/21343311http://purl.uniprot.org/core/author"Liu S.N."xsd:string
http://purl.uniprot.org/citations/21343311http://purl.uniprot.org/core/author"Ou B."xsd:string
http://purl.uniprot.org/citations/21343311http://purl.uniprot.org/core/author"Ou B."xsd:string
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