| http://purl.uniprot.org/citations/21343338 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21343338 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21343338 | http://www.w3.org/2000/01/rdf-schema#comment | "Regulation of tau exon 10 splicing plays an important role in tauopathy. One of the cis elements regulating tau alternative splicing is a stem-loop structure at the 5' splice site of tau exon 10. The RNA helicase(s) modulating this stem-loop structure was unknown. We searched for splicing regulators interacting with this stem-loop region using an RNA affinity pulldown-coupled mass spectrometry approach and identified DDX5/RNA helicase p68 as an activator of tau exon 10 splicing. The activity of p68 in stimulating tau exon 10 inclusion is dependent on RBM4, an intronic splicing activator. RNase H cleavage and U1 protection assays suggest that p68 promotes conformational change of the stem-loop structure, thereby increasing the access of U1snRNP to the 5' splice site of tau exon 10. This study reports the first RNA helicase interacting with a stem-loop structure at the splice site and regulating alternative splicing in a helicase-dependent manner. Our work uncovers a previously unknown function of p68 in regulating tau exon 10 splicing. Furthermore, our experiments reveal functional interaction between two splicing activators for tau exon 10, p68 binding at the stem-loop region and RBM4 interacting with the intronic splicing enhancer region."xsd:string |
| http://purl.uniprot.org/citations/21343338 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.01149-10"xsd:string |
| http://purl.uniprot.org/citations/21343338 | http://purl.org/dc/terms/identifier | "doi:10.1128/mcb.01149-10"xsd:string |
| http://purl.uniprot.org/citations/21343338 | http://purl.uniprot.org/core/author | "Chen S."xsd:string |
| http://purl.uniprot.org/citations/21343338 | http://purl.uniprot.org/core/author | "Chen S."xsd:string |
| http://purl.uniprot.org/citations/21343338 | http://purl.uniprot.org/core/author | "Chen X."xsd:string |
| http://purl.uniprot.org/citations/21343338 | http://purl.uniprot.org/core/author | "Chen X."xsd:string |
| http://purl.uniprot.org/citations/21343338 | http://purl.uniprot.org/core/author | "Liu Z."xsd:string |
| http://purl.uniprot.org/citations/21343338 | http://purl.uniprot.org/core/author | "Liu Z."xsd:string |
| http://purl.uniprot.org/citations/21343338 | http://purl.uniprot.org/core/author | "Zhou X."xsd:string |
| http://purl.uniprot.org/citations/21343338 | http://purl.uniprot.org/core/author | "Zhou X."xsd:string |
| http://purl.uniprot.org/citations/21343338 | http://purl.uniprot.org/core/author | "Shi C."xsd:string |
| http://purl.uniprot.org/citations/21343338 | http://purl.uniprot.org/core/author | "Shi C."xsd:string |
| http://purl.uniprot.org/citations/21343338 | http://purl.uniprot.org/core/author | "Ray P."xsd:string |
| http://purl.uniprot.org/citations/21343338 | http://purl.uniprot.org/core/author | "Ray P."xsd:string |
| http://purl.uniprot.org/citations/21343338 | http://purl.uniprot.org/core/author | "Fushimi K."xsd:string |
| http://purl.uniprot.org/citations/21343338 | http://purl.uniprot.org/core/author | "Fushimi K."xsd:string |
| http://purl.uniprot.org/citations/21343338 | http://purl.uniprot.org/core/author | "Kar A."xsd:string |
| http://purl.uniprot.org/citations/21343338 | http://purl.uniprot.org/core/author | "Kar A."xsd:string |
| http://purl.uniprot.org/citations/21343338 | http://purl.uniprot.org/core/author | "Wu J.Y."xsd:string |
| http://purl.uniprot.org/citations/21343338 | http://purl.uniprot.org/core/author | "Wu J.Y."xsd:string |
| http://purl.uniprot.org/citations/21343338 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21343338 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |