| http://purl.uniprot.org/citations/21361779 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21361779 | http://www.w3.org/2000/01/rdf-schema#comment | "We have previously shown that the Ser15-phosphorylated p53 phosphoform, p53(Ser15), can localize at sites of ionizing radiation-induced DNA damage. In this study, we hypothesized that the non-specific DNA binding domain (NSDBD) of the p53 carboxy-terminus (C-terminus) mediates chromatin anchoring at sites of DNA damage to interact with two key mediators of the DNA damage response (DDR): ATM and 53BP1. Exogenous YFP-p53 fusion constructs expressing C-terminus deletion mutants of p53 were transfected into p53-null H1299 cells and tracked by microscopy and biochemistry to determine relative chromatin-binding pre- and postirradiation. We observed that exogenous YFP-p53(WT) and YFP-p53(Δ367-393) associated with ATM(Ser1981) and 53BP1 in the nuclear, chromatin-bound fractions after DNA damage. Of interest, YFP-p53(Δ1-299) fusion proteins, which lack transcriptional trans-activation and the Ser15-residue, bound to ATM(Ser1981) but not to 53BP1. In support of these data, we used subnuclear UV-microbeam and immunoprecipitation analyses of irradiated normal human fibroblasts (HDFs) that confirmed an interaction between endogenous p53 and ATM or 53BP1. Based on these observations, we propose a model whereby a pre-existing pool of p53 responds immediately to radiation-induced DNA damage using the C-terminus to spatially facilitate protein-protein interactions and the DDR at sites of DNA damage."xsd:string |
| http://purl.uniprot.org/citations/21361779 | http://purl.org/dc/terms/identifier | "doi:10.1667/rr2084.1"xsd:string |
| http://purl.uniprot.org/citations/21361779 | http://purl.uniprot.org/core/author | "Harding S.M."xsd:string |
| http://purl.uniprot.org/citations/21361779 | http://purl.uniprot.org/core/author | "Bristow R.G."xsd:string |
| http://purl.uniprot.org/citations/21361779 | http://purl.uniprot.org/core/author | "Al Rashid S.T."xsd:string |
| http://purl.uniprot.org/citations/21361779 | http://purl.uniprot.org/core/author | "Law C."xsd:string |
| http://purl.uniprot.org/citations/21361779 | http://purl.uniprot.org/core/author | "Coackley C."xsd:string |
| http://purl.uniprot.org/citations/21361779 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21361779 | http://purl.uniprot.org/core/name | "Radiat Res"xsd:string |
| http://purl.uniprot.org/citations/21361779 | http://purl.uniprot.org/core/pages | "588-598"xsd:string |
| http://purl.uniprot.org/citations/21361779 | http://purl.uniprot.org/core/title | "Protein-protein interactions occur between p53 phosphoforms and ATM and 53BP1 at sites of exogenous DNA damage."xsd:string |
| http://purl.uniprot.org/citations/21361779 | http://purl.uniprot.org/core/volume | "175"xsd:string |
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