| http://purl.uniprot.org/citations/21362622 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21362622 | http://www.w3.org/2000/01/rdf-schema#comment | "Sld3 is essential for the initiation of DNA replication, but Sld3 does not travel with a replication fork. GINS binds to Cdc45 and Mcm2-7 to form the replication fork helicase in eukaryotes. We purified Sld3, Cdc45, GINS, and Mcm2-7 and studied their interaction and assembly into complexes. Sld3 binds tightly to Cdc45 in the presence or absence of cyclin-dependent kinase activity. Furthermore, Sld3 binds tightly to the Mcm2-7 complex, and a ternary complex forms among Cdc45, Mcm2-7, and Sld3, with a 1:1:1 stoichiometry (CMS complex). GINS binds directly to Mcm2-7, and GINS competes with Sld3 for Mcm2-7 binding. GINS also binds directly to Cdc45, and GINS competes with Sld3 for Cdc45 binding. Cdc45, Mcm2-7, and GINS form a ternary complex with a stoichiometry of 1:1:1 (CMG complex). Size exclusion data reveal that when Sld3, Cdc45, Mcm2-7, and GINS are added together, the result is a mixture of CMS and CMG complexes. The data suggest that GINS and Sld3 compete with one another for Mcm2-7 and Cdc45 binding. Our results are consistent with a model wherein GINS trades places with Sld3 at a replication origin, contributing to the activation of the replication fork helicase."xsd:string |
| http://purl.uniprot.org/citations/21362622 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m111.218305"xsd:string |
| http://purl.uniprot.org/citations/21362622 | http://purl.uniprot.org/core/author | "Kaplan D.L."xsd:string |
| http://purl.uniprot.org/citations/21362622 | http://purl.uniprot.org/core/author | "Bruck I."xsd:string |
| http://purl.uniprot.org/citations/21362622 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21362622 | http://purl.uniprot.org/core/name | "J Biol Chem"xsd:string |
| http://purl.uniprot.org/citations/21362622 | http://purl.uniprot.org/core/pages | "14157-14167"xsd:string |
| http://purl.uniprot.org/citations/21362622 | http://purl.uniprot.org/core/title | "GINS and Sld3 compete with one another for Mcm2-7 and Cdc45 binding."xsd:string |
| http://purl.uniprot.org/citations/21362622 | http://purl.uniprot.org/core/volume | "286"xsd:string |
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