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http://purl.uniprot.org/citations/21370452http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/21370452http://www.w3.org/2000/01/rdf-schema#comment"

Scope

The aim of this research was to explore whether the tea-polyphenol (-)-epigallocatechin-3-gallate (EGCG) could be used as a potential agent for blocking smoking (nicotine, Nic)- or hormone (estradiol, E2)-induced breast cancer cell proliferation through inhibition of a common signaling pathway.

Methods and results

To explore whether Nic (>0.1 μM, 24 h) and E2 (>1 nM, 24 h) significantly increased α9-nicotinic acetylcholine (α9-nicotinic acetylcholine receptor (nAChR)) mRNA and protein expression levels, real-time PCR and immunoblotting analysis experiments were performed in human breast cancer (MCF-7) cells. Luciferase promoter activity experiment was performed to test the α9-nAChR promoter activity affected by Nic, E2 or EGCG. The results indicate that treatment with EGCG (1 μM) profoundly decreases Nic- and E2-induced MCF-7 proliferation by down regulating α9-nAChR expression. The α9-nAChR promoter activity is significantly induced by 24-h treatment with Nic (10 μM) or E2 (10 nM) (>1.8 and ∼2.3-fold, respectively) in MCF-7 cells. Pretreatment with EGCG eliminated the Nic- and E2-induced α9-nAChR promoter-dependent luciferase activity. We further demonstrate that combined treatment with EGCG profoundly inhibits [3H]-Nic/ α9-nAChR binding activity in breast cancer cells.

Conclusions

We found that the EGCG could be used as an agent for blocking smoking (Nic)- or hormone (E2)-induced breast cancer cell proliferation by inhibiting of α9-nAChR signaling pathway. This study reveals the novel antitumor mechanisms of EGCG, and these results may have significant applications for chemopreventive purposes in human breast cancer."xsd:string
http://purl.uniprot.org/citations/21370452http://purl.org/dc/terms/identifier"doi:10.1002/mnfr.201000254"xsd:string
http://purl.uniprot.org/citations/21370452http://purl.uniprot.org/core/author"Chang Y.J."xsd:string
http://purl.uniprot.org/citations/21370452http://purl.uniprot.org/core/author"Chang C.H."xsd:string
http://purl.uniprot.org/citations/21370452http://purl.uniprot.org/core/author"Chang H.W."xsd:string
http://purl.uniprot.org/citations/21370452http://purl.uniprot.org/core/author"Huang C.S."xsd:string
http://purl.uniprot.org/citations/21370452http://purl.uniprot.org/core/author"Chen C.S."xsd:string
http://purl.uniprot.org/citations/21370452http://purl.uniprot.org/core/author"Lee C.H."xsd:string
http://purl.uniprot.org/citations/21370452http://purl.uniprot.org/core/author"Ho Y.S."xsd:string
http://purl.uniprot.org/citations/21370452http://purl.uniprot.org/core/author"Wu C.H."xsd:string
http://purl.uniprot.org/citations/21370452http://purl.uniprot.org/core/author"Pan M.H."xsd:string
http://purl.uniprot.org/citations/21370452http://purl.uniprot.org/core/author"Chen L.C."xsd:string
http://purl.uniprot.org/citations/21370452http://purl.uniprot.org/core/author"Tu S.H."xsd:string
http://purl.uniprot.org/citations/21370452http://purl.uniprot.org/core/author"Ku C.Y."xsd:string
http://purl.uniprot.org/citations/21370452http://purl.uniprot.org/core/author"Wei P.L."xsd:string
http://purl.uniprot.org/citations/21370452http://purl.uniprot.org/core/author"Ho C.T."xsd:string
http://purl.uniprot.org/citations/21370452http://purl.uniprot.org/core/date"2011"xsd:gYear
http://purl.uniprot.org/citations/21370452http://purl.uniprot.org/core/name"Mol Nutr Food Res"xsd:string
http://purl.uniprot.org/citations/21370452http://purl.uniprot.org/core/pages"455-466"xsd:string
http://purl.uniprot.org/citations/21370452http://purl.uniprot.org/core/title"Tea polyphenol (-)-epigallocatechin-3-gallate inhibits nicotine- and estrogen-induced alpha9-nicotinic acetylcholine receptor upregulation in human breast cancer cells."xsd:string
http://purl.uniprot.org/citations/21370452http://purl.uniprot.org/core/volume"55"xsd:string
http://purl.uniprot.org/citations/21370452http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/21370452
http://purl.uniprot.org/citations/21370452http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/21370452
http://purl.uniprot.org/uniprot/#_Q9UGM1-mappedCitation-21370452http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/21370452
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