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http://purl.uniprot.org/citations/2137461http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/2137461http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/2137461http://www.w3.org/2000/01/rdf-schema#comment"The receptor for mouse interferon gamma (IFN-gamma) was purified from detergent-solubilized plasma membranes of EL-4, a thymoma cell line which expresses a high number of receptors on its cell surface. The purification was carried out by immunoaffinity chromatography using an anti-receptor monoclonal antibody. The purified receptor was subjected to NH2-terminal sequence analysis as well as sequencing of endopeptidase-generated peptides. One of the peptides was found to be identical to a portion of the published amino acid sequence of the human IFN-gamma receptor deduced from cDNA. This information was utilized to construct a mixed-sequence oligodeoxynucleotide probe which permitted the isolation of a full-length cDNA clone coding for the mouse IFN-gamma receptor. The mouse IFN-gamma receptor cDNA is comprised of 105 base pairs of the 5'-untranslated region, an open reading frame coding for a 477-amino acid serine-rich protein having calculated Mr 52,276, and a 3'-untranslated region of 539 base pairs. The receptor is first synthesized as a pre-protein from which a 25-amino acid signal peptide is cleaved. The receptor contains a hydrophobic transmembrane portion near the center of the molecule. Northern blot analysis of various cell lines showed that each contained a single 2.0-kilobase mRNA. A direct correlation between the amount of IFN-gamma receptor mRNA and the level of receptor expressed on the cell surface was observed. The mouse and human IFN-gamma receptors are structurally similar, showing 51% over-all homology in amino acid sequence. Mouse IFN-gamma receptor cDNA when inserted in a mammalian shuttle vector and transfected into COS-7 monkey cells was able to direct the expression of specific binding activity for mouse IFN-gamma."xsd:string
http://purl.uniprot.org/citations/2137461http://purl.org/dc/terms/identifier"doi:10.1016/s0021-9258(19)39703-0"xsd:string
http://purl.uniprot.org/citations/2137461http://purl.org/dc/terms/identifier"doi:10.1016/s0021-9258(19)39703-0"xsd:string
http://purl.uniprot.org/citations/2137461http://purl.uniprot.org/core/author"Appella E."xsd:string
http://purl.uniprot.org/citations/2137461http://purl.uniprot.org/core/author"Appella E."xsd:string
http://purl.uniprot.org/citations/2137461http://purl.uniprot.org/core/author"Tanaka S."xsd:string
http://purl.uniprot.org/citations/2137461http://purl.uniprot.org/core/author"Tanaka S."xsd:string
http://purl.uniprot.org/citations/2137461http://purl.uniprot.org/core/author"Yuhki N."xsd:string
http://purl.uniprot.org/citations/2137461http://purl.uniprot.org/core/author"Yuhki N."xsd:string
http://purl.uniprot.org/citations/2137461http://purl.uniprot.org/core/author"Landolfo S."xsd:string
http://purl.uniprot.org/citations/2137461http://purl.uniprot.org/core/author"Landolfo S."xsd:string
http://purl.uniprot.org/citations/2137461http://purl.uniprot.org/core/author"Moore S.K."xsd:string
http://purl.uniprot.org/citations/2137461http://purl.uniprot.org/core/author"Moore S.K."xsd:string
http://purl.uniprot.org/citations/2137461http://purl.uniprot.org/core/author"Cofano F."xsd:string
http://purl.uniprot.org/citations/2137461http://purl.uniprot.org/core/author"Cofano F."xsd:string
http://purl.uniprot.org/citations/2137461http://purl.uniprot.org/core/date"1990"xsd:gYear
http://purl.uniprot.org/citations/2137461http://purl.uniprot.org/core/date"1990"xsd:gYear
http://purl.uniprot.org/citations/2137461http://purl.uniprot.org/core/name"J. Biol. Chem."xsd:string
http://purl.uniprot.org/citations/2137461http://purl.uniprot.org/core/name"J. Biol. Chem."xsd:string
http://purl.uniprot.org/citations/2137461http://purl.uniprot.org/core/pages"4064-4071"xsd:string
http://purl.uniprot.org/citations/2137461http://purl.uniprot.org/core/pages"4064-4071"xsd:string
http://purl.uniprot.org/citations/2137461http://purl.uniprot.org/core/title"Affinity purification, peptide analysis, and cDNA sequence of the mouse interferon gamma receptor."xsd:string
http://purl.uniprot.org/citations/2137461http://purl.uniprot.org/core/title"Affinity purification, peptide analysis, and cDNA sequence of the mouse interferon gamma receptor."xsd:string
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