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| http://purl.uniprot.org/citations/21392508 | http://www.w3.org/2000/01/rdf-schema#comment | "Yeast prion [PSI(+)] is a self-perpetuating amyloid of the translational termination factor Sup35. Although [PSI(+)] propagation is modulated by heat shock proteins (Hsps), high temperature was previously reported to have little or no effect on [PSI(+)]. Our results show that short-term exposure of exponentially growing yeast culture to mild heat shock, followed by immediate resumption of growth, leads to [PSI(+)] destabilization, sometimes persisting for several cell divisions after heat shock. Prion loss occurring in the first division after heat shock is preferentially detected in a daughter cell, indicating the impairment of prion segregation that results in asymmetric prion distribution between a mother cell and a bud. Longer heat shock or prolonged incubation in the absence of nutrients after heat shock led to [PSI(+)] recovery. Both prion destabilization and recovery during heat shock depend on protein synthesis. Maximal prion destabilization coincides with maximal imbalance between Hsp104 and other Hsps such as Hsp70-Ssa. Deletions of individual SSA genes increase prion destabilization and/or counteract recovery. The dynamics of prion aggregation during destabilization and recovery are consistent with the notion that efficient prion fragmentation and segregation require a proper balance between Hsp104 and other (e.g., Hsp70-Ssa) chaperones. In contrast to heat shock, [PSI(+)] destabilization by osmotic stressors does not always depend on cell proliferation and/or protein synthesis, indicating that different stresses may impact the prion via different mechanisms. Our data demonstrate that heat stress causes asymmetric prion distribution in a cell division and confirm that the effects of Hsps on prions are physiologically relevant."xsd:string |
| http://purl.uniprot.org/citations/21392508 | http://purl.org/dc/terms/identifier | "doi:10.1016/j.jmb.2011.02.034"xsd:string |
| http://purl.uniprot.org/citations/21392508 | http://purl.uniprot.org/core/author | "Chernoff Y.O."xsd:string |
| http://purl.uniprot.org/citations/21392508 | http://purl.uniprot.org/core/author | "Birchmore J.L."xsd:string |
| http://purl.uniprot.org/citations/21392508 | http://purl.uniprot.org/core/author | "Newnam G.P."xsd:string |
| http://purl.uniprot.org/citations/21392508 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21392508 | http://purl.uniprot.org/core/name | "J Mol Biol"xsd:string |
| http://purl.uniprot.org/citations/21392508 | http://purl.uniprot.org/core/pages | "432-448"xsd:string |
| http://purl.uniprot.org/citations/21392508 | http://purl.uniprot.org/core/title | "Destabilization and recovery of a yeast prion after mild heat shock."xsd:string |
| http://purl.uniprot.org/citations/21392508 | http://purl.uniprot.org/core/volume | "408"xsd:string |
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