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http://purl.uniprot.org/citations/21402795 | http://www.w3.org/2000/01/rdf-schema#comment | "In this study, we examined the involvement of endogenous abscisic acid (ABA) in methyl jasmonate (MeJA)-induced stomatal closure using an inhibitor of ABA biosynthesis, fluridon (FLU), and an ABA-deficient Arabidopsis (Arabidopsis thaliana) mutant, aba2-2. We found that pretreatment with FLU inhibited MeJA-induced stomatal closure but not ABA-induced stomatal closure in wild-type plants. The aba2-2 mutation impaired MeJA-induced stomatal closure but not ABA-induced stomatal closure. We also investigated the effects of FLU and the aba2-2 mutation on cytosolic free calcium concentration ([Ca(2+)](cyt)) in guard cells using a Ca(2+)-reporter fluorescent protein, Yellow Cameleon 3.6. In wild-type guard cells, FLU inhibited MeJA-induced [Ca(2+)](cyt) elevation but not ABA-induced [Ca(2+)](cyt) elevation. The aba2-2 mutation did not affect ABA-elicited [Ca(2+)](cyt) elevation but suppressed MeJA-induced [Ca(2+)](cyt) elevation. We also tested the effects of the aba2-2 mutation and FLU on the expression of MeJA-inducible VEGETATIVE STORAGE PROTEIN1 (VSP1). In the aba2-2 mutant, MeJA did not induce VSP1 expression. In wild-type leaves, FLU inhibited MeJA-induced VSP1 expression. Pretreatment with ABA at 0.1 μm, which is not enough concentration to evoke ABA responses in the wild type, rescued the observed phenotypes of the aba2-2 mutant. Finally, we found that in wild-type leaves, MeJA stimulates the expression of 9-CIS-EPOXYCAROTENOID DIOXYGENASE3, which encodes a crucial enzyme in ABA biosynthesis. These results suggest that endogenous ABA could be involved in MeJA signal transduction and lead to stomatal closure in Arabidopsis guard cells."xsd:string |
http://purl.uniprot.org/citations/21402795 | http://purl.org/dc/terms/identifier | "doi:10.1104/pp.111.172254"xsd:string |
http://purl.uniprot.org/citations/21402795 | http://purl.uniprot.org/core/author | "Nakamura Y."xsd:string |
http://purl.uniprot.org/citations/21402795 | http://purl.uniprot.org/core/author | "Munemasa S."xsd:string |
http://purl.uniprot.org/citations/21402795 | http://purl.uniprot.org/core/author | "Murata Y."xsd:string |
http://purl.uniprot.org/citations/21402795 | http://purl.uniprot.org/core/author | "Mori I.C."xsd:string |
http://purl.uniprot.org/citations/21402795 | http://purl.uniprot.org/core/author | "Hossain M.A."xsd:string |
http://purl.uniprot.org/citations/21402795 | http://purl.uniprot.org/core/author | "Uraji M."xsd:string |
http://purl.uniprot.org/citations/21402795 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
http://purl.uniprot.org/citations/21402795 | http://purl.uniprot.org/core/name | "Plant Physiol"xsd:string |
http://purl.uniprot.org/citations/21402795 | http://purl.uniprot.org/core/pages | "430-438"xsd:string |
http://purl.uniprot.org/citations/21402795 | http://purl.uniprot.org/core/title | "Involvement of endogenous abscisic acid in methyl jasmonate-induced stomatal closure in Arabidopsis."xsd:string |
http://purl.uniprot.org/citations/21402795 | http://purl.uniprot.org/core/volume | "156"xsd:string |
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