| http://purl.uniprot.org/citations/21455770 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21455770 | http://www.w3.org/2000/01/rdf-schema#comment | "Heat shock factor 1 (HSF1) is the major heat shock transcription factor and plays an essential role in mediating the cellular response to physiological and environmental stress. We found that LPS-induced expression of the granulocyte-colony stimulating factor (G-CSF) gene was upregulated in HSF1 knock-out (HSF1(-/-)) mice using a gene array. In order to determine whether and how HSF1 regulates the induced expression of G-CSF, mRNA, and protein levels of G-CSF were detected by Northern blotting and ELISA, the promoter of G-CSF was analyzed with an online transcription element search system and the transcriptional activity of the G-CSF promoter was analyzed by EMSA and a reporter gene assay. The results showed that transcription and protein secretion of G-CSF induced by LPS are both inhibited by HSF1. Three high affinity binding sites for NF-IL6/CCAAT enhancer binding protein beta, but no heat shock element, were identified in the core promoter of G-CSF. The DNA-binding capability of NF-IL6 to the G-CSF promoter was reinforced by LPS but not influenced by heat shock or HSF1. However, HSF1 was observed to bind to the binding sites of NF-IL6 in the G-CSF promoter. The transcriptional activity of the G-CSF promoter was enhanced by LPS or NF-IL6 and inhibited by HSF1 in a dose dependent manner. We conclude that HSF1 regulates expression of G-CSF through binding to the NF-IL6-binding element."xsd:string |
| http://purl.uniprot.org/citations/21455770 | http://purl.org/dc/terms/identifier | "doi:10.1007/s11010-010-0624-1"xsd:string |
| http://purl.uniprot.org/citations/21455770 | http://purl.uniprot.org/core/author | "Liang Q."xsd:string |
| http://purl.uniprot.org/citations/21455770 | http://purl.uniprot.org/core/author | "Liu M."xsd:string |
| http://purl.uniprot.org/citations/21455770 | http://purl.uniprot.org/core/author | "Wang Q."xsd:string |
| http://purl.uniprot.org/citations/21455770 | http://purl.uniprot.org/core/author | "Zhang H."xsd:string |
| http://purl.uniprot.org/citations/21455770 | http://purl.uniprot.org/core/author | "Zhang L."xsd:string |
| http://purl.uniprot.org/citations/21455770 | http://purl.uniprot.org/core/author | "Yang M."xsd:string |
| http://purl.uniprot.org/citations/21455770 | http://purl.uniprot.org/core/author | "Xiao X."xsd:string |
| http://purl.uniprot.org/citations/21455770 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21455770 | http://purl.uniprot.org/core/name | "Mol Cell Biochem"xsd:string |
| http://purl.uniprot.org/citations/21455770 | http://purl.uniprot.org/core/pages | "11-17"xsd:string |
| http://purl.uniprot.org/citations/21455770 | http://purl.uniprot.org/core/title | "HSF1 regulates expression of G-CSF through the binding element for NF-IL6/CCAAT enhancer binding protein beta."xsd:string |
| http://purl.uniprot.org/citations/21455770 | http://purl.uniprot.org/core/volume | "352"xsd:string |
| http://purl.uniprot.org/citations/21455770 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/21455770 |
| http://purl.uniprot.org/citations/21455770 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/21455770 |
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| http://purl.uniprot.org/uniprot/#_A0A075F891-mappedCitation-21455770 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21455770 |
| http://purl.uniprot.org/uniprot/#_Q0VB73-mappedCitation-21455770 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21455770 |
| http://purl.uniprot.org/uniprot/#_P09920-mappedCitation-21455770 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21455770 |
| http://purl.uniprot.org/uniprot/#_P28033-mappedCitation-21455770 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21455770 |
| http://purl.uniprot.org/uniprot/#_P38532-mappedCitation-21455770 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21455770 |