| http://purl.uniprot.org/citations/21467216 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21467216 | http://www.w3.org/2000/01/rdf-schema#comment | "Herpesvirus type 1 (HSV-1) based oncolytic vectors arise as a promising therapeutic alternative for neoplastic diseases including hepatocellular carcinoma. However, the mechanisms mediating the host cell response to such treatments are not completely known. It is well established that HSV-1 infection induces functional and structural alterations in the nucleus of the host cell. In the present work, we have used gel-based and shotgun proteomic strategies to elucidate the signaling pathways impaired in the nucleus of human hepatoma cells (Huh7) upon HSV-1 Cgal(+) infection. Both approaches allowed the identification of differential proteins suggesting impairment of cell functions involved in many aspects of host-virus interaction such as transcription regulation, mRNA processing, and mRNA splicing. Based on our proteomic data and additional functional studies, cellular protein quaking content (QKI) increases 4 hours postinfection (hpi), when viral immediate-early genes such as ICP4 and ICP27 could be also detected. Depletion of QKI expression by small interfering RNA results in reduction of viral immediate-early protein levels, subsequent decrease in early and late viral protein content, and a reduction in the viral yield indicating that QKI directly interferes with viral replication. In particular, HSV-1 Cgal(+) induces a transient increase in quaking I-5 isoform (QKI-5) levels, in parallel with an enhancement of p27(Kip1) protein content. Moreover, immunofluorescence microscopy showed an early nuclear redistribution of QKI-5, shuttling from the nucleus to the cytosol and colocalizing with nectin-1 in cell to cell contact regions at 16-24 hpi. This evidence sheds new light on mechanisms mediating hepatoma cell response to HSV-1 vectors highlighting QKI as a central molecular mediator."xsd:string |
| http://purl.uniprot.org/citations/21467216 | http://purl.org/dc/terms/identifier | "doi:10.1074/mcp.m111.009126"xsd:string |
| http://purl.uniprot.org/citations/21467216 | http://purl.uniprot.org/core/author | "Prieto J."xsd:string |
| http://purl.uniprot.org/citations/21467216 | http://purl.uniprot.org/core/author | "Greco A."xsd:string |
| http://purl.uniprot.org/citations/21467216 | http://purl.uniprot.org/core/author | "Corrales F.J."xsd:string |
| http://purl.uniprot.org/citations/21467216 | http://purl.uniprot.org/core/author | "Epstein A.L."xsd:string |
| http://purl.uniprot.org/citations/21467216 | http://purl.uniprot.org/core/author | "Sanchez J.C."xsd:string |
| http://purl.uniprot.org/citations/21467216 | http://purl.uniprot.org/core/author | "Segura V."xsd:string |
| http://purl.uniprot.org/citations/21467216 | http://purl.uniprot.org/core/author | "Sanchez-Quiles V."xsd:string |
| http://purl.uniprot.org/citations/21467216 | http://purl.uniprot.org/core/author | "Santamaria E."xsd:string |
| http://purl.uniprot.org/citations/21467216 | http://purl.uniprot.org/core/author | "Mora M.I."xsd:string |
| http://purl.uniprot.org/citations/21467216 | http://purl.uniprot.org/core/author | "Dayon L."xsd:string |
| http://purl.uniprot.org/citations/21467216 | http://purl.uniprot.org/core/author | "Foschini M.G."xsd:string |
| http://purl.uniprot.org/citations/21467216 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21467216 | http://purl.uniprot.org/core/name | "Mol Cell Proteomics"xsd:string |
| http://purl.uniprot.org/citations/21467216 | http://purl.uniprot.org/core/pages | "M111.009126"xsd:string |
| http://purl.uniprot.org/citations/21467216 | http://purl.uniprot.org/core/title | "HSV-1 Cgal+ infection promotes quaking RNA binding protein production and induces nuclear-cytoplasmic shuttling of quaking I-5 isoform in human hepatoma cells."xsd:string |
| http://purl.uniprot.org/citations/21467216 | http://purl.uniprot.org/core/volume | "10"xsd:string |
| http://purl.uniprot.org/citations/21467216 | http://www.w3.org/2004/02/skos/core#exactMatch | http://purl.uniprot.org/pubmed/21467216 |
| http://purl.uniprot.org/citations/21467216 | http://xmlns.com/foaf/0.1/primaryTopicOf | https://pubmed.ncbi.nlm.nih.gov/21467216 |
| http://purl.uniprot.org/uniprot/#_B4DHR6-mappedCitation-21467216 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21467216 |
| http://purl.uniprot.org/uniprot/#_Q8WY44-mappedCitation-21467216 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21467216 |
| http://purl.uniprot.org/uniprot/#_Q96PU8-mappedCitation-21467216 | http://www.w3.org/1999/02/22-rdf-syntax-ns#object | http://purl.uniprot.org/citations/21467216 |
| http://purl.uniprot.org/uniprot/B4DHR6 | http://purl.uniprot.org/core/mappedCitation | http://purl.uniprot.org/citations/21467216 |