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http://purl.uniprot.org/citations/21481304http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/21481304http://www.w3.org/2000/01/rdf-schema#comment"

Aim

To establish a ubiquitously expressed PD-L1 transgenic mouse model and evaluate its recovery of motor function after spinal cord injury.

Methods

Clone and sequence the complete mouse PD-L1 cDNA and construct the pCAG-PD-L1 transgenic vector by inserting the PD-L1 cDNA into the pCAGGS vector. The PD-L1 transgenic (TgPD-L1) mice were established by pronuclear micro-injection with fertilized eggs from C57BL/6 mice and the genotypes were confirmed by PCR with tail genomic DNA. The expression of PD-L1 on T and B lymphocytes from mouse spleen were detected by flow cytometry. The expression of PD-L1 in peripheral tissues was displayed by immunohistochemistry. The expression level of PD-L1 in spinal tissue was evaluated by RT-PCR. The recovery of motor function was analyzed by Basso-Beattie-Bresnahan(BBB) locomotion testing system at 3, 7, 14, 21, 28 and 35 day after spinal severe crush with forceps in mice.

Results

Three lines of TgPD-L1 mice in C57BL/6 background were generated and the exogenous PD-L1 gene can be heritable steadily to offsprings. PD-L1 was highly exppressed in spinal tissue, peripheral tissues, T and B lymphocytes using RT-PCR, immunohistochemistry and flow cytometry respectively in TgPD-L1 mice. The BBB scores were obviously higher at 21 day post-injury in TgPD-L1 than those of in WT mice (P<0.05).

Conclusion

The TgPD-L1 mice whose background are C57BL/6 were established successfully and high expression level of PD-L1 in tissues promotes locomotion recovery after spinal cord injury in TgPD-L1 mice."xsd:string
http://purl.uniprot.org/citations/21481304http://purl.uniprot.org/core/author"Tang L."xsd:string
http://purl.uniprot.org/citations/21481304http://purl.uniprot.org/core/author"Wang J."xsd:string
http://purl.uniprot.org/citations/21481304http://purl.uniprot.org/core/author"Zhao Y."xsd:string
http://purl.uniprot.org/citations/21481304http://purl.uniprot.org/core/author"Yu C.Y."xsd:string
http://purl.uniprot.org/citations/21481304http://purl.uniprot.org/core/author"Feng R."xsd:string
http://purl.uniprot.org/citations/21481304http://purl.uniprot.org/core/author"Liu F.F."xsd:string
http://purl.uniprot.org/citations/21481304http://purl.uniprot.org/core/author"Ju G."xsd:string
http://purl.uniprot.org/citations/21481304http://purl.uniprot.org/core/author"Guo H.M."xsd:string
http://purl.uniprot.org/citations/21481304http://purl.uniprot.org/core/date"2011"xsd:gYear
http://purl.uniprot.org/citations/21481304http://purl.uniprot.org/core/name"Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi"xsd:string
http://purl.uniprot.org/citations/21481304http://purl.uniprot.org/core/pages"357-359"xsd:string
http://purl.uniprot.org/citations/21481304http://purl.uniprot.org/core/title"[Establishment of PD-L1 transgenic mouse model and recovery of the motion after spinal cord injury]."xsd:string
http://purl.uniprot.org/citations/21481304http://purl.uniprot.org/core/volume"27"xsd:string
http://purl.uniprot.org/citations/21481304http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/21481304
http://purl.uniprot.org/citations/21481304http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/21481304
http://purl.uniprot.org/uniprot/#_Q3U472-mappedCitation-21481304http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/21481304
http://purl.uniprot.org/uniprot/#_Q9EP73-mappedCitation-21481304http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/21481304
http://purl.uniprot.org/uniprot/Q3U472http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/21481304
http://purl.uniprot.org/uniprot/Q9EP73http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/21481304