| http://purl.uniprot.org/citations/21483673 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21483673 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21483673 | http://www.w3.org/2000/01/rdf-schema#comment | "BackgroundVoltage-dependent K(+) channels (Kv) mediate repolarisation of β-cell action potentials, and thereby abrogate insulin secretion. The role of the Kv1.1 K(+) channel in this process is however unclear. We tested for presence of Kv1.1 in different species and tested for a functional role of Kv1.1 by assessing pancreatic islet function in BALB/cByJ (wild-type) and megencephaly (mceph/mceph) mice, the latter having a deletion in the Kv1.1 gene.Methodology/principal findingsKv1.1 expression was detected in islets from wild-type mice, SD rats and humans, and expression of truncated Kv1.1 was detected in mceph/mceph islets. Full-length Kv1.1 protein was present in islets from wild-type mice, but, as expected, not in those from mceph/mceph mice. Kv1.1 expression was localized to the β-cell population and also to α- and δ-cells, with evidence of over-expression of truncated Kv1.1 in mceph/mceph islets. Blood glucose, insulin content, and islet morphology were normal in mceph/mceph mice, but glucose-induced insulin release from batch-incubated islets was (moderately) higher than that from wild-type islets. Reciprocal blocking of Kv1.1 by dendrotoxin-K increased insulin secretion from wild-type but not mceph/mceph islets. Glucose-induced action potential duration, as well as firing frequency, was increased in mceph/mceph mouse β-cells. This duration effect on action potential in β-cells from mceph/mceph mice was mimicked by dendrotoxin-K in β-cells from wild-type mice. Observations concerning the effects of both the mceph mutation, and of dendrotoxin-K, on glucose-induced insulin release were confirmed in pancreatic islets from Kv1.1 null mice.Conclusion/significanceKv1.1 channels are expressed in the β-cells of several species, and these channels can influence glucose-stimulated insulin release."xsd:string |
| http://purl.uniprot.org/citations/21483673 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0018213"xsd:string |
| http://purl.uniprot.org/citations/21483673 | http://purl.org/dc/terms/identifier | "doi:10.1371/journal.pone.0018213"xsd:string |
| http://purl.uniprot.org/citations/21483673 | http://purl.uniprot.org/core/author | "Ma Z."xsd:string |
| http://purl.uniprot.org/citations/21483673 | http://purl.uniprot.org/core/author | "Ma Z."xsd:string |
| http://purl.uniprot.org/citations/21483673 | http://purl.uniprot.org/core/author | "Berglund E."xsd:string |
| http://purl.uniprot.org/citations/21483673 | http://purl.uniprot.org/core/author | "Berglund E."xsd:string |
| http://purl.uniprot.org/citations/21483673 | http://purl.uniprot.org/core/author | "Portwood N."xsd:string |
| http://purl.uniprot.org/citations/21483673 | http://purl.uniprot.org/core/author | "Portwood N."xsd:string |
| http://purl.uniprot.org/citations/21483673 | http://purl.uniprot.org/core/author | "Falkmer S."xsd:string |
| http://purl.uniprot.org/citations/21483673 | http://purl.uniprot.org/core/author | "Falkmer S."xsd:string |
| http://purl.uniprot.org/citations/21483673 | http://purl.uniprot.org/core/author | "Sundler F."xsd:string |
| http://purl.uniprot.org/citations/21483673 | http://purl.uniprot.org/core/author | "Sundler F."xsd:string |
| http://purl.uniprot.org/citations/21483673 | http://purl.uniprot.org/core/author | "Almgren M."xsd:string |
| http://purl.uniprot.org/citations/21483673 | http://purl.uniprot.org/core/author | "Almgren M."xsd:string |
| http://purl.uniprot.org/citations/21483673 | http://purl.uniprot.org/core/author | "Bjorklund A."xsd:string |
| http://purl.uniprot.org/citations/21483673 | http://purl.uniprot.org/core/author | "Bjorklund A."xsd:string |
| http://purl.uniprot.org/citations/21483673 | http://purl.uniprot.org/core/author | "Branstrom R."xsd:string |
| http://purl.uniprot.org/citations/21483673 | http://purl.uniprot.org/core/author | "Branstrom R."xsd:string |
| http://purl.uniprot.org/citations/21483673 | http://purl.uniprot.org/core/author | "Forsberg L.E."xsd:string |
| http://purl.uniprot.org/citations/21483673 | http://purl.uniprot.org/core/author | "Forsberg L.E."xsd:string |
| http://purl.uniprot.org/citations/21483673 | http://purl.uniprot.org/core/author | "Lavebratt C."xsd:string |
| http://purl.uniprot.org/citations/21483673 | http://purl.uniprot.org/core/author | "Lavebratt C."xsd:string |