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http://purl.uniprot.org/citations/21485539http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/21485539http://www.w3.org/2000/01/rdf-schema#comment"

Objective

To investigate the mRNA and protein expression levels of cysteine-rich secretory protein 2 (CRISP2) in the sperm of asthenospermia patients, and explore their relationship with sperm motility and related molecular mechanism.

Methods

We collected 78 semen samples from adult male patients with asthenospermia and another 70 from healthy volunteers as controls. We extracted total RNA and total protein from the sperm following purification of the sperm by Percoll gradient centrifugation, and detected the relative expressions of CRISP2 mRNA and protein in the two groups by RT-PCR, SYBR Green real-time PCR and Western blot.

Results

The expression of CRISP2 mRNA was down-regulated by 4.3 times and that of the CRISP2 protein by 1.71 times in the asthenospermia patients, significantly lower than in the normal control group (P < 0.05).

Conclusion

The down-regulation of CRISP2 mRNA and protein expressions in the sperm of asthenospermia patients may be closely related with decreased sperm motility, which suggests that CRISP2 may serve as a potential molecular target for the research of asthenospermia."xsd:string
http://purl.uniprot.org/citations/21485539http://purl.uniprot.org/core/author"Mao X.M."xsd:string
http://purl.uniprot.org/citations/21485539http://purl.uniprot.org/core/author"Chen S.M."xsd:string
http://purl.uniprot.org/citations/21485539http://purl.uniprot.org/core/author"Guo W.B."xsd:string
http://purl.uniprot.org/citations/21485539http://purl.uniprot.org/core/author"Chu Q.J."xsd:string
http://purl.uniprot.org/citations/21485539http://purl.uniprot.org/core/author"Yu Q.F."xsd:string
http://purl.uniprot.org/citations/21485539http://purl.uniprot.org/core/author"Feng C.Q."xsd:string
http://purl.uniprot.org/citations/21485539http://purl.uniprot.org/core/author"Jing X.W."xsd:string
http://purl.uniprot.org/citations/21485539http://purl.uniprot.org/core/author"Xing R.W."xsd:string
http://purl.uniprot.org/citations/21485539http://purl.uniprot.org/core/author"Zhou Q.Z."xsd:string
http://purl.uniprot.org/citations/21485539http://purl.uniprot.org/core/date"2011"xsd:gYear
http://purl.uniprot.org/citations/21485539http://purl.uniprot.org/core/name"Zhonghua Nan Ke Xue"xsd:string
http://purl.uniprot.org/citations/21485539http://purl.uniprot.org/core/pages"203-207"xsd:string
http://purl.uniprot.org/citations/21485539http://purl.uniprot.org/core/title"[Expressions of cysteine-rich secretory protein 2 in asthenospermia]."xsd:string
http://purl.uniprot.org/citations/21485539http://purl.uniprot.org/core/volume"17"xsd:string
http://purl.uniprot.org/citations/21485539http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/21485539
http://purl.uniprot.org/citations/21485539http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/21485539
http://purl.uniprot.org/uniprot/#_A0A024RD74-mappedCitation-21485539http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/21485539
http://purl.uniprot.org/uniprot/#_P16562-mappedCitation-21485539http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/21485539
http://purl.uniprot.org/uniprot/A0A024RD74http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/21485539
http://purl.uniprot.org/uniprot/P16562http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/21485539