| http://purl.uniprot.org/citations/21489993 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21489993 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21489993 | http://www.w3.org/2000/01/rdf-schema#comment | "Histone modifications and DNA methylation represent two layers of heritable epigenetic information that regulate eukaryotic chromatin structure and gene activity. UHRF1 is a unique factor that bridges these two layers; it is required for maintenance DNA methylation at hemimethylated CpG sites, which are specifically recognized through its SRA domain and also interacts with histone H3 trimethylated on lysine 9 (H3K9me3) in an unspecified manner. Here we show that UHRF1 contains a tandem Tudor domain (TTD) that recognizes H3 tail peptides with the heterochromatin-associated modification state of trimethylated lysine 9 and unmodified lysine 4 (H3K4me0/K9me3). Solution NMR and crystallographic data reveal the TTD simultaneously recognizes H3K9me3 through a conserved aromatic cage in the first Tudor subdomain and unmodified H3K4 within a groove between the tandem subdomains. The subdomains undergo a conformational adjustment upon peptide binding, distinct from previously reported mechanisms for dual histone mark recognition. Mutant UHRF1 protein deficient for H3K4me0/K9me3 binding shows altered localization to heterochromatic chromocenters and fails to reduce expression of a target gene, p16(INK4A), when overexpressed. Our results demonstrate a novel recognition mechanism for the combinatorial readout of histone modification states associated with gene silencing and add to the growing evidence for coordination of, and cross-talk between, the modification states of H3K4 and H3K9 in regulation of gene expression."xsd:string |
| http://purl.uniprot.org/citations/21489993 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m111.234104"xsd:string |
| http://purl.uniprot.org/citations/21489993 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.m111.234104"xsd:string |
| http://purl.uniprot.org/citations/21489993 | http://purl.org/dc/terms/identifier | "doi:10.1074/jbc.M111.234104"xsd:string |
| http://purl.uniprot.org/citations/21489993 | http://purl.uniprot.org/core/author | "Arrowsmith C.H."xsd:string |
| http://purl.uniprot.org/citations/21489993 | http://purl.uniprot.org/core/author | "Arrowsmith C.H."xsd:string |
| http://purl.uniprot.org/citations/21489993 | http://purl.uniprot.org/core/author | "Duan S."xsd:string |
| http://purl.uniprot.org/citations/21489993 | http://purl.uniprot.org/core/author | "Duan S."xsd:string |
| http://purl.uniprot.org/citations/21489993 | http://purl.uniprot.org/core/author | "Wang Y.X."xsd:string |
| http://purl.uniprot.org/citations/21489993 | http://purl.uniprot.org/core/author | "Wang Y.X."xsd:string |
| http://purl.uniprot.org/citations/21489993 | http://purl.uniprot.org/core/author | "Xue S."xsd:string |
| http://purl.uniprot.org/citations/21489993 | http://purl.uniprot.org/core/author | "Xue S."xsd:string |
| http://purl.uniprot.org/citations/21489993 | http://purl.uniprot.org/core/author | "Walker J.R."xsd:string |
| http://purl.uniprot.org/citations/21489993 | http://purl.uniprot.org/core/author | "Walker J.R."xsd:string |
| http://purl.uniprot.org/citations/21489993 | http://purl.uniprot.org/core/author | "Chedin F."xsd:string |
| http://purl.uniprot.org/citations/21489993 | http://purl.uniprot.org/core/author | "Chedin F."xsd:string |
| http://purl.uniprot.org/citations/21489993 | http://purl.uniprot.org/core/author | "Dhe-Paganon S."xsd:string |
| http://purl.uniprot.org/citations/21489993 | http://purl.uniprot.org/core/author | "Dhe-Paganon S."xsd:string |
| http://purl.uniprot.org/citations/21489993 | http://purl.uniprot.org/core/author | "Allali-Hassani A."xsd:string |
| http://purl.uniprot.org/citations/21489993 | http://purl.uniprot.org/core/author | "Allali-Hassani A."xsd:string |
| http://purl.uniprot.org/citations/21489993 | http://purl.uniprot.org/core/author | "Zuo X."xsd:string |
| http://purl.uniprot.org/citations/21489993 | http://purl.uniprot.org/core/author | "Zuo X."xsd:string |
| http://purl.uniprot.org/citations/21489993 | http://purl.uniprot.org/core/author | "Avvakumov G.V."xsd:string |