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http://purl.uniprot.org/citations/21492422http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/21492422http://www.w3.org/2000/01/rdf-schema#comment"

Introduction

Systemic lupus erythematosus (SLE) is an autoimmune disease with chronic or episodic inflammation in many different organ systems, activation of leukocytes and production of pro-inflammatory cytokines. The heterodimer of the cytosolic calcium-binding proteins S100A8 and S100A9 (S100A8/A9) is secreted by activated polymorphonuclear neutrophils (PMNs) and monocytes and serves as a serum marker for several inflammatory diseases. Furthermore, S100A8 and S100A9 have many pro-inflammatory properties such as binding to Toll-like receptor 4 (TLR4). In this study we investigated if aberrant cell surface S100A8/A9 could be seen in SLE and if plasmacytoid dendritic cells (pDCs) could synthesize S100A8/A9.

Methods

Flow cytometry, confocal microscopy and real-time PCR of flow cytometry-sorted cells were used to measure cell surface S100A8/A9, intracellular S100A8/A9 and mRNA levels of S100A8 and S100A9, respectively.

Results

Cell surface S100A8/A9 was detected on all leukocyte subpopulations investigated except for T cells. By confocal microscopy, real-time PCR and stimulation assays, we could demonstrate that pDCs, monocytes and PMNs could synthesize S100A8/A9. Furthermore, pDC cell surface S100A8/A9 was higher in patients with active disease as compared to patients with inactive disease. Upon immune complex stimulation, pDCs up-regulated the cell surface S100A8/A9. SLE patients had also increased serum levels of S100A8/A9.

Conclusions

Patients with SLE had increased cell surface S100A8/A9, which could be important in amplification and persistence of inflammation. Importantly, pDCs were able to synthesize S100A8/A9 proteins and up-regulate the cell surface expression upon immune complex-stimulation. Thus, S100A8/A9 may be a potent target for treatment of inflammatory diseases such as SLE."xsd:string
http://purl.uniprot.org/citations/21492422http://purl.org/dc/terms/identifier"doi:10.1186/ar3314"xsd:string
http://purl.uniprot.org/citations/21492422http://purl.uniprot.org/core/author"Lood C."xsd:string
http://purl.uniprot.org/citations/21492422http://purl.uniprot.org/core/author"Leanderson T."xsd:string
http://purl.uniprot.org/citations/21492422http://purl.uniprot.org/core/author"Sturfelt G."xsd:string
http://purl.uniprot.org/citations/21492422http://purl.uniprot.org/core/author"Truedsson L."xsd:string
http://purl.uniprot.org/citations/21492422http://purl.uniprot.org/core/author"Gullstrand B."xsd:string
http://purl.uniprot.org/citations/21492422http://purl.uniprot.org/core/author"Ivars F."xsd:string
http://purl.uniprot.org/citations/21492422http://purl.uniprot.org/core/author"Kallberg E."xsd:string
http://purl.uniprot.org/citations/21492422http://purl.uniprot.org/core/author"Bengtsson A.A."xsd:string
http://purl.uniprot.org/citations/21492422http://purl.uniprot.org/core/author"Stenstrom M."xsd:string
http://purl.uniprot.org/citations/21492422http://purl.uniprot.org/core/author"Tyden H."xsd:string
http://purl.uniprot.org/citations/21492422http://purl.uniprot.org/core/date"2011"xsd:gYear
http://purl.uniprot.org/citations/21492422http://purl.uniprot.org/core/name"Arthritis Res Ther"xsd:string
http://purl.uniprot.org/citations/21492422http://purl.uniprot.org/core/pages"R60"xsd:string
http://purl.uniprot.org/citations/21492422http://purl.uniprot.org/core/title"Protein synthesis of the pro-inflammatory S100A8/A9 complex in plasmacytoid dendritic cells and cell surface S100A8/A9 on leukocyte subpopulations in systemic lupus erythematosus."xsd:string
http://purl.uniprot.org/citations/21492422http://purl.uniprot.org/core/volume"13"xsd:string
http://purl.uniprot.org/citations/21492422http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/21492422
http://purl.uniprot.org/citations/21492422http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/21492422
http://purl.uniprot.org/uniprot/#_B2R4M6-mappedCitation-21492422http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/21492422
http://purl.uniprot.org/uniprot/#_P05109-mappedCitation-21492422http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/21492422
http://purl.uniprot.org/uniprot/#_P06702-mappedCitation-21492422http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/21492422
http://purl.uniprot.org/uniprot/B2R4M6http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/21492422
http://purl.uniprot.org/uniprot/P06702http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/21492422
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