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http://purl.uniprot.org/citations/21497337http://www.w3.org/1999/02/22-rdf-syntax-ns#typehttp://purl.uniprot.org/core/Journal_Citation
http://purl.uniprot.org/citations/21497337http://www.w3.org/2000/01/rdf-schema#comment"

Objective

To evaluate whether the protective effect of melatonin on H2O2-induced caspase activation and DNA fragmentation depends on the interaction between melatonin and its surface receptors.

Design

Laboratory study.

Setting

Center for assisted human reproduction at a Spanish hospital.

Patient(s)

Twenty-one healthy donors.

Intervention(s)

Human spermatozoa were treated with increasing concentrations of hydrogen peroxide (H2O2; 1 μM, 10 μM, 100 μM, 1 mM) and preincubated with 1 mM melatonin.

Main outcomes measure(s)

Activation of caspase-3 and -9 as well as DNA fragmentation were examined by fluorescence methods.

Result(s)

Our findings showed that H2O2 induced a significant increase in caspase-9 and caspase-3, which was dose independent. Conversely, pretreatment with melatonin reduced H2O2-mediated caspase activation in a dose-dependent way. Moreover, the antiapoptotic effects of melatonin in ejaculated human spermatozoa may involve membrane melatonin receptor MT1. In addition, we found that the survival-promoting pathway extracellular signal-regulated kinase (ERK) is likely to have a role in the protective actions of melatonin in ejaculated human spermatozoa. Finally, we confirmed these results further by demonstrating that melatonin prevention of H2O2-induced DNA fragmentation is dependent on both MT1 receptor and ERK signaling.

Conclusion(s)

These results indicate that the stimulation with melatonin triggers a set of events culminating in cell death prevention in ejaculated human spermatozoa."xsd:string
http://purl.uniprot.org/citations/21497337http://purl.org/dc/terms/identifier"doi:10.1016/j.fertnstert.2011.03.063"xsd:string
http://purl.uniprot.org/citations/21497337http://purl.uniprot.org/core/author"Ortiz A."xsd:string
http://purl.uniprot.org/citations/21497337http://purl.uniprot.org/core/author"Garcia J.F."xsd:string
http://purl.uniprot.org/citations/21497337http://purl.uniprot.org/core/author"Espino J."xsd:string
http://purl.uniprot.org/citations/21497337http://purl.uniprot.org/core/author"Rodriguez A.B."xsd:string
http://purl.uniprot.org/citations/21497337http://purl.uniprot.org/core/author"Pariente J.A."xsd:string
http://purl.uniprot.org/citations/21497337http://purl.uniprot.org/core/author"Bejarano I."xsd:string
http://purl.uniprot.org/citations/21497337http://purl.uniprot.org/core/author"Lozano G.M."xsd:string
http://purl.uniprot.org/citations/21497337http://purl.uniprot.org/core/author"Monllor F."xsd:string
http://purl.uniprot.org/citations/21497337http://purl.uniprot.org/core/date"2011"xsd:gYear
http://purl.uniprot.org/citations/21497337http://purl.uniprot.org/core/name"Fertil Steril"xsd:string
http://purl.uniprot.org/citations/21497337http://purl.uniprot.org/core/pages"2290-2296"xsd:string
http://purl.uniprot.org/citations/21497337http://purl.uniprot.org/core/title"Melatonin protects human spermatozoa from apoptosis via melatonin receptor- and extracellular signal-regulated kinase-mediated pathways."xsd:string
http://purl.uniprot.org/citations/21497337http://purl.uniprot.org/core/volume"95"xsd:string
http://purl.uniprot.org/citations/21497337http://www.w3.org/2004/02/skos/core#exactMatchhttp://purl.uniprot.org/pubmed/21497337
http://purl.uniprot.org/citations/21497337http://xmlns.com/foaf/0.1/primaryTopicOfhttps://pubmed.ncbi.nlm.nih.gov/21497337
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http://purl.uniprot.org/uniprot/#_Q8WZ72-mappedCitation-21497337http://www.w3.org/1999/02/22-rdf-syntax-ns#objecthttp://purl.uniprot.org/citations/21497337
http://purl.uniprot.org/uniprot/Q29RX9http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/21497337
http://purl.uniprot.org/uniprot/P48039http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/21497337
http://purl.uniprot.org/uniprot/Q8WZ72http://purl.uniprot.org/core/mappedCitationhttp://purl.uniprot.org/citations/21497337