| http://purl.uniprot.org/citations/21505417 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21505417 | http://www.w3.org/1999/02/22-rdf-syntax-ns#type | http://purl.uniprot.org/core/Journal_Citation |
| http://purl.uniprot.org/citations/21505417 | http://www.w3.org/2000/01/rdf-schema#comment | "The cilium is an important organelle that is found on many eukaryotic cells, where it serves essential functions in motility, sensory reception and signalling. Intraflagellar transport (IFT) is a vital process for the formation and maintenance of cilia. We have determined the crystal structure of Chlamydomonas reinhardtii IFT25/27, an IFT sub-complex, at 2.6 Å resolution. IFT25 and IFT27 interact via a conserved interface that we verify biochemically using structure-guided mutagenesis. IFT27 displays the fold of Rab-like small guanosine triphosphate hydrolases (GTPases), binds GTP and GDP with micromolar affinity and has very low intrinsic GTPase activity, suggesting that it likely requires a GTPase-activating protein (GAP) for robust GTP turnover. A patch of conserved surface residues contributed by both IFT25 and IFT27 is found adjacent to the GTP-binding site and could mediate the binding to other IFT proteins as well as to a potential GAP. These results provide the first step towards a high-resolution structural understanding of the IFT complex."xsd:string |
| http://purl.uniprot.org/citations/21505417 | http://purl.org/dc/terms/identifier | "doi:10.1038/emboj.2011.110"xsd:string |
| http://purl.uniprot.org/citations/21505417 | http://purl.org/dc/terms/identifier | "doi:10.1038/emboj.2011.110"xsd:string |
| http://purl.uniprot.org/citations/21505417 | http://purl.uniprot.org/core/author | "Lorentzen E."xsd:string |
| http://purl.uniprot.org/citations/21505417 | http://purl.uniprot.org/core/author | "Lorentzen E."xsd:string |
| http://purl.uniprot.org/citations/21505417 | http://purl.uniprot.org/core/author | "Basquin C."xsd:string |
| http://purl.uniprot.org/citations/21505417 | http://purl.uniprot.org/core/author | "Basquin C."xsd:string |
| http://purl.uniprot.org/citations/21505417 | http://purl.uniprot.org/core/author | "Taschner M."xsd:string |
| http://purl.uniprot.org/citations/21505417 | http://purl.uniprot.org/core/author | "Taschner M."xsd:string |
| http://purl.uniprot.org/citations/21505417 | http://purl.uniprot.org/core/author | "Bhogaraju S."xsd:string |
| http://purl.uniprot.org/citations/21505417 | http://purl.uniprot.org/core/author | "Bhogaraju S."xsd:string |
| http://purl.uniprot.org/citations/21505417 | http://purl.uniprot.org/core/author | "Morawetz M."xsd:string |
| http://purl.uniprot.org/citations/21505417 | http://purl.uniprot.org/core/author | "Morawetz M."xsd:string |
| http://purl.uniprot.org/citations/21505417 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21505417 | http://purl.uniprot.org/core/date | "2011"xsd:gYear |
| http://purl.uniprot.org/citations/21505417 | http://purl.uniprot.org/core/name | "EMBO J."xsd:string |
| http://purl.uniprot.org/citations/21505417 | http://purl.uniprot.org/core/name | "EMBO J."xsd:string |
| http://purl.uniprot.org/citations/21505417 | http://purl.uniprot.org/core/pages | "1907-1918"xsd:string |
| http://purl.uniprot.org/citations/21505417 | http://purl.uniprot.org/core/pages | "1907-1918"xsd:string |
| http://purl.uniprot.org/citations/21505417 | http://purl.uniprot.org/core/title | "Crystal structure of the intraflagellar transport complex 25/27."xsd:string |
| http://purl.uniprot.org/citations/21505417 | http://purl.uniprot.org/core/title | "Crystal structure of the intraflagellar transport complex 25/27."xsd:string |
| http://purl.uniprot.org/citations/21505417 | http://purl.uniprot.org/core/volume | "30"xsd:string |
| http://purl.uniprot.org/citations/21505417 | http://purl.uniprot.org/core/volume | "30"xsd:string |